京尼平巩膜交联对兔形觉剥夺性近视形成的抑制作用

Inhibitory effect of scleral crosslinking using genipin on form-deprivation myopia in rabbits

目的探讨京尼平巩膜交联对形觉剥夺性近视模型兔眼生物学参数及巩膜生物力学的影响。方法取出生后14 d健康新西兰幼兔60只,均以右眼为实验眼。应用随机数字表法平均分为3个组,每组20只。空白对照组双眼不予处理,近视模型组右眼行单纯眼睑缝合,京尼平注射组右眼行眼睑缝合联合京尼平结膜下注射。眼睑缝合后60 d拆开实验眼眼睑缝线,测量双眼屈光度、玻璃体腔长度和眼轴长度,取实验眼鼻上1:00位和颞下7:00位巩膜条带,测量其厚度及弹性模量、蠕变率、极限应力和极限应变。结果京尼平注射组、近视模型组和空白对照组屈光度分别为(2.50±1.38)、(0.33±0.52)和(2.08±0.52)D,眼轴长度分别为(15.33±0.82)、(15.83±0.41)和(15.00±0.43)mm,玻璃体腔长度变化值分别为(1.50±0.79)、(2.59±0.83)和(1.48±0.66)mm,玻璃体腔与眼轴比值分别为0.46±0.02、0.51±0.02和0.47±0.02。近视模型组屈光度明显低于空白对照组和京尼平注射组,玻璃体腔长度变化值和玻璃体腔长度与眼轴长度比值均明显高于空白对照组和京尼平注射组,近视模型组眼轴长度明显长于空白对照组,差异均有统计学意义(均P<0.05)。京尼平注射组巩膜条带弹性模量为(9.10±3.12)MPa,极限应力为(1.42±0.57)MPa,均明显高于空白对照组的(6.98±3.30)MPa和(1.15±0.57)MPa以及近视模型组的(6.25±3.17)MPa和(0.82±0.38)MPa;京尼平注射组巩膜条带极限应变为(20.99±5.60)%,明显小于空白对照组的(25.08±6.35)%以及近视模型组的(27.78±8.20)%,差异均有统计学意义(均P<0.05)。结论后Tenon囊下注射京尼平巩膜胶原交联能够提高兔眼巩膜的生物力学强度,并能有效阻止兔眼形觉剥夺性近视的发展,为防治近视进展提供了新的思路。

Objective To investigate the effects of scleral crosslinking using genipin on ocular biological parameters and scleral biomechanics of form-deprivation myopia rabbits.Methods Sixty healthy New Zealand rabbits of 14 days old were collected.The right eyes were selected as experimental eye.The rabbits were randomly divided into three groups:control group with no treatment;myopia model group with eyelid suture procedure performed on the right eye;genipin injection group with eyelid suture procedure performed on the right eye combined with subconjunctival injection of genipin.The suture was removed 60 days after the eyelid suture procedure.The diopter,length of vitreous cavity,and axial length were measured.The sclera at 1:00 and 7:00 position of the experimental eye was used to make a scleral strip.The thickness,elastic modulus,creep rate,ultimate stress and ultimate strain of the sclera were measured.This study was approved by the animal experimental Ethics Committee of Bethune International Peace Hospital(2018-ky-09).Results The diopters of genipin injection group,myopia model group and control group were(2.50±1.38),(0.33±0.52)and(2.08±0.52)D,respectively,the axial lengths of the three groups were(15.33±0.82),(15.83±0.41)and(15.00±0.43)mm,respectively;the changes in vitreous cavity lengths were(1.50±0.79),(2.59±0.83)and(1.48±0.66)mm,respectively;and the ratios of vitreous cavity length to axial length were 0.46±0.02,0.51±0.02 and 0.47±0.02,respectively.The diopter in myopia model group was significantly lower than those in control group and genipin injection group,the axial length in myopia model group was significantly longer than that in control group,the change in vitreous cavity lengths and ratio of vitreous cavity length to axial length in myopia model group were significantly higher than those in control group and genipin injection group,the axial length in myopia model group was significantly longer than that in control group,the differences were statistically significant(all at P<0.05).The elasticity modulus was(9.10±3.12)MPa and ultimate stress was(1.42±0.57)MPa in genipin injection group,which were significantly higher than(6.98±3.30)MPa and(1.15±0.57)MPa in control group,and(6.25±3.17)MPa and(0.82±0.38)MPa in myopia model group(all at P<0.05).The ultimate strain was(20.99±5.60)%in genipin injection group,which was significantly lower than(25.08±6.35)%in control group and(27.78±8.20)%in myopia model group,with significant differences between them(both at P<0.05).Conclusions Posterior sub-Tenon capsule injection of genipin for collagen crosslinking in the sclera increases the biomechanical strength of sclera and effectively prevents the development of form-deprivation myopia in rabbits,which provides a new idea for the prevention and treatment of myopia in the future.