应用流式细胞术测定绢毛苣属4种8居群基因组大小

Estimation of genome size from eight populations of four species of Soroseris Stebbins by flow cytometry

以绢毛苣属(Soroseris)植物肉菊(S.umbrella(Franch.)Lipsch)、绢毛苣(S.glomerata(Decne.)Stebbins)、羽裂绢毛苣(S.hirsuta(Anth.)Shih)和金沙绢毛菊(S.gillii(S.Moore)Stebbins)4个种的8个居群为材料,采用WPB和Galbraith s buffer两种裂解液分别处理样品,运用流式细胞术测定DNA含量,并使用常规染色体压片法确定其细胞倍性。结果显示:两种裂解液中,最适于绢毛苣属植物DNA含量测定的裂解液为WPB;4个物种中,羽裂绢毛苣的基因组最小;细胞倍性水平对1Cx-值的影响较小。此外,本研究发现可采用种子萌发形成的幼苗代替难以获取的植物幼嫩叶片作为实验材料。

In this study,eight populations of four Soroseris Stebbins species were used as experimental materials.The DNA content was determined by flow cytometry based on WPB and Galbraith s buffer and ploidy was determined by traditional chromosome tableting.Results showed that,after comparing the two dissociation buffers,WPB was found to be the most suitable for Soroseris.In addition,S.hirsuta(Anth.)Shih had the smallest genome and the polyploidy level had little effect on the 1Cx-value in the four species.Furthermore,this study also found that seedlings formed by seed germination could be used as experimental materials instead of young leaves that are difficult to obtain.