HDAC2与乙酰化P53k320在胃癌细胞中的表达及对其疗效的评价

EXPRESSION OF HDAC2 AND ACETYLATED P53k320 IN GASTRIC CANCER CELLS AND EVALUATION OF ITS THERAPEUTIC EFFECT

目的 探讨胃癌的早期诊断及疗效评价指标。方法 选取胃癌患者130例,取其癌组织和癌旁组织,检测其HDAC2及乙酰化P53^k320阳性表达情况,HDAC2mRNA、HDAC2蛋白、乙酰化P53^k320蛋白表达量。结果 胃癌组织HDAC2阳性表达率高于癌旁组织,乙酰化P53^k320阳性率低于癌旁组织(P <0.01)。胃癌细胞中HDAC2mRNA、HDAC2蛋白表达量均高于癌旁细胞,乙酰化P53k320蛋白表达量低于癌旁细胞(P <0.01)。CR、PR、SD、PD组患者癌组织中HDAC2蛋白表达量呈增高趋势,乙酰化P53k320蛋白表达量呈降低趋势(P <0.01)。结论胃癌细胞中HDAC2、乙酰化P53^k320蛋白表达情况与治疗效果密切相关。

Objective To investigate the early diagnosis and evaluation of curative effect of gastric cancer.Methods A total of 130 patients with gastric cancer were enrolled.The cancer tissues and adjacent tissues were obtained.the expression of HDAC2 and acetylated P53^k320,HDAC2 mRNA,HDAC2 protein and acetylated P53^k320 protein were detected.Results The positive expression rate of HDAC2 in gastric cancer tissues was higher than that in adjacent tissues.The positive rate of acetylated P53^k320 was lower than that in adjacent tissues(P<0.01).The expression of HDAC2 mRNA and HDAC2 protein in gastric cancer cells were higher than those in adjacent cells,and the expression of acetylated P53 k320 protein was lower than that in adjacent cells(P<0.01).The expression of HDAC2 protein in the cancer tissues of CR,PR,SD and PD groups increased,and the expression of acetylated P53^k320 protein decreased(P<0.01).Conclusion The expression of HDAC2 and acetylated P53^k320 protein in gastric cancer cells is closely related to the treatment effect.