建立正常成人甲状旁腺素参考区间的研究

The Establishment of Reference Range of Human Parathyroid Hormone in Normal Population

目的建立本地区健康成年人群的甲状旁腺素的参考区间,为检测甲状旁腺功能提供依据。方法选取2018年6月至2018年9月在本院进行健康体检的健康人群样本(参考个体),采用化学发光免疫分析技术检测血清甲状旁腺素,应用SPSS 21.0统计软件对所收集数据进行统计分析,采用双侧参考限建立参考区间及对参考区间进行验证,并比较分析年龄、性别的差异。结果共纳入120例参考个体,血清甲状旁腺素的检测结果呈正态分布,参考区间为10.78~101.19pg/mL;参考区间验证结果显示20例参考个体其测定值均在参考区间范围内;不同性别分组统计结果显示血清甲状旁腺素无性别差异(P=0.710);不同年龄分组比较结果显示血清甲状旁腺素水平无年龄差异(P=0.347)。结论本地区医疗机构可采用10.78~01.19pg/mL作为正常成人血清甲状旁腺素参考区间,或建立本实验室的参考区间。

Objective We aimed to establish the reference range of parathyroid hormone in healthy population in this area,and to provide evidence for the detection of parathyroid function.Methods The prospective study of healthy individuals underwent a health checkup in our hospital from June,2018 to September,2018 was conducted.We used Chemoluminescence immunoassay to detect parathyroid hormone in serum of 120 selected healthy subjects from physical examination.SPSS 21.0 statistical software was used to analyze the collected data.95%confidence interval of bilateral boundaries was used to establish the reference range and reference range validation was also performed.Results from groups with different ages and different gender were compared.Results Serum parathyroid hormones were skewed in 120 healthy subjects.The normal reference range of parathyroid hormone was 10.78-101.19pg/mL and the reference range was then validated.The difference between the two sex groups was not statistically significant.The results of parathyroid hormone among different age groups showed that the level of parathyroid hormone in the group of>45 years old was similar with that in the group of≤45 years old(P=0.347).Conclusion 10.78-101.19pg/mL can be used by local medical institutions as the reference range of serum parathyroid hormone in normal population,or the reference interval of our own laboratory can be established.