西藏地区藏族宫颈癌患者自身抗体特点研究

A Study on Characteristics of Autoantibodies in Tibetan Cervical Cancer Patients in Tibet Region

目的探讨西藏地区藏族宫颈癌患者自身抗体特点及其与宫颈癌的相关性。方法选取2017年3月至2019年3月于我院确诊的藏族宫颈癌患者72例,另选择同时期我院藏族健康体检人群60例为对照组。采取免疫组化法检测血清抗核抗体(ANA),用酶联免疫吸附法检测血浆BIRC5、MYC、P16自身抗体和血清Koc、Survivin、CycBI、CycE、CDK2自身抗体,用受试者工作曲线(ROC)分析BIRC5、MYC、P16诊断宫颈癌的价值。结果宫颈癌组ANA阳性率、抗SP抗体阳性率、Koc、Survivin、CycBI、CycE、CDK2自身抗体阳性率均高于对照组(P<0.05),血浆BIRC5、MYC、P16自身抗体水平均高于对照组(P<0.05);ROC曲线分析结果显示:BIRC5、MYC、P16自身抗体联合检测诊断宫颈癌的AUC值、灵敏度、约登指数均显著高于单一指标检测(P<0.05);Koc、Survivin、CycBI、CycE、CDK2自身抗体联合检测诊断宫颈癌的灵敏度、约登指数均显著高于单一指标检测(P<0.05)。结论西藏地区藏族宫颈癌与ANA以及BIRC5、MYC、P16、Koc、Survivin、CycBI、CycE、CDK2自身抗体有一定关联性,自身抗体联合检测可提高诊断宫颈癌的准确性。

Objective To explore the characteristics of autoantibodies in Tibetan cervical cancer patients in Tibet region and their correlations with cervical cancer.Methods Seventy-two Tibetan patients with cervical cancer diagnosed in our hospital from March,2017 to September,2019 were selected for the study,while 60 Tibetan people with healthy physical examination results in our hospital were selected as the control group.Serum antinuclear antibody(ANA)was detected by immunohistochemistry,and plasma BIRC5,MYC,P16 autoantibodies and serum Koc,Survivin,CycBI,CycE,CDK2 autoantibodies were detected by enzyme-linked immunosorbent assay(ELISA).Receiver operating characteristic curve(ROC)was used to analyze the values of BIRC5,MYC and P16 in the diagnosis of cervical cancer.Results The ANA positive rate,anti-SP antibody positive rate and positive rates of Koc,Survivin,CycBI,CycE and CDK2 autoantibodies in the cervical cancer group were higher than those in the control group(all P<0.05),and the levels of plasma BIRC5,MYC and P16 autoantibodies in the cervical cancer group were higher than those in the control group(all P<0.05).ROC curve analysis showed that the AUC value,sensitivity and Yoden index of diagnosis of cervical cancer by BIRC5,MYC and P16 autoantibodies were significantly higher than those of single index(all P<0.05).The sensitivity and Yoden index of combined detection of Survivin,CycBI,CycE and CDK2 autoantibodies were significantly higher than those of single index(P<0.05).Conclusion Cervical cancer among Tibetan patients in Tibet region has a certain correlation with ANA and BIRC5,MYC,P16,Koc,Survivin,CycBI,CycE and CDK2 autoantibodies.The combination of autoantibodies can improve the accuracy of cervical cancer diagnosis.