Rakicidin B1发酵提取液活性炭脱色工艺的研究

Study on the activated carbon decolorization process of rakicidin B1 extract

目的研究rakicidin B1发酵提取液的脱色工艺条件,提高纯化后rakicidin B1样品的质量。方法以UV和HPLC为检测方法,以脱色率和rakicidin B1保留率为指标,先通过单因素实验考察活性炭用量、脱色时间、脱色温度以及pH值对指标的影响。在单因素基础上,采用4因素3水平正交试验分析各因素与两个指标的关系。结果 Rakicidin B1发酵提取液采用活性炭脱色最佳条件为活性炭用量0.5%、脱色时间40min、脱色温度50℃、pH7.0,在该条件下活性炭脱色率达74.53%,rakicidin B1保留率为85.66%。结论该脱色工艺简单可靠,脱色效果较好,适合于工业化生产。

Objective To discuss the process of bleaching rakicidin B1 extracting solution with activated carbon.Methods Based on the single factor experiment,the influence of four main factors including the amount of activated carbon,bleaching time,temperature,and pH were researched.Orthogonal test design was applied to screen out the optimal conditions for the decolorization of rakicidin B1 extract.The decolorization ratio and the retention ratio of rakicidin B1 were used as indexes,which were determined by ultraviolet spectrophotometer and HPLC.Results The experimental results showed that the optimal bleaching conditions were as follows:rakicidin B1 was decolorized at 50℃for 40 min with the addition of 0.5%of activated carbon adjusted to pH7.0,bringing forth a decolorization rate of 74.53%and a retention ratio of 85.66%.Conclusion This method is simple and available for the commercial process.