3种传统方法对不同珊瑚表面积测量的适用性及其校准方法——以3D扫描技术为基准

Applicability and calibration methods of three traditional surface area measurement methods for different coral species——based on 3D scanning technology

造礁石珊瑚的表面积是珊瑚生物学和珊瑚礁生态学研究中重要的参数,因其形态结构复杂,准确测量十分困难。由于测量方法的不同,导致不同珊瑚之间的重要指标无法被直接比较,因此系统比较不同的表面积测量方法十分必要。以块状的普哥滨珊瑚Porites pukoensis和分枝状的鹿角杯形珊瑚Pocillopora damicornis为研究对象,以3D扫描技术测量的珊瑚表面积作为标准值,对比石蜡包埋法、锡箔纸包裹法以及简单几何近似法3种传统方法测量珊瑚表面积的准确度;讨论了不同的表面积测量方法对于不同形态结构珊瑚的适用性;并通过简单回归分析,校准传统测量方法的测量准确度。研究结果表明,无论在普哥滨珊瑚还是鹿角杯形珊瑚中,简单几何近似法的测量准确度最高(72.71%和94.52%),石蜡包埋法其次(68.86%和83.08%),锡箔纸包裹法最低(65.27%和58.07%)。简单回归分析结果表明,除了锡箔纸包裹法测量普哥滨珊瑚表面积与3D扫描测量结果的相关性较低(r^2=0.76)外,其他传统测量方法与3D扫描测量结果均具有较强相关性(r^2>0.95)。因此,研究认为可通过简单回归分析构建回归方程对传统测量方法进行校准,以提高传统方法的测量准确度。

The surface area of scleractinian corals is an important reference parameter required for various researchs of coral biology and coral reef ecology.However,due to its complex morphological structure,the accurate measurement of coral surface area remains difficult.The diversity of measurement methods leads to the unreachable direct comparison of important indicators between different coral species.Thus,it is necessary to make a systematic comparison of different surface area measurement methods in different coral species.Regarding coral surface area measured by 3D scanning technique as standard,this study investigated the accuracy of three traditional measurement methods(wax coating,aluminium foil and simple geometry),discussed the applicability of different methods for corals in different morphology,and calibrated the accuracy of traditional measurement methods by simple regression analysis of two scleractinian corals Porites pukoensis(massive)and Pocillopora damicornis(branching).Results showed that the accuracy of simple geometry method was the highest(72.71%and 94.52%),followed by that of wax coating method(68.86%and 83.08%),and the accuracy of aluminium foil method is the lowest(65.27%and 58.07%)for P.pukoensis and P.damicornis,respectively.A strong correlation between 3D scanning method and each of the three traditional measurement methods(r^2>0.95)was revealed by simple regression analysis,except for the surface area of P.pukoensis measured by the aluminium foil method(r^2=0.762).Overall,to improve the accuracy of traditional coral surface area measurement methods,regression equations constructed by simple regression analysis can be used for calibration.