茯茶多糖提取工艺优化及其体外抗氧化和对益生菌生长的影响

Optimization of extraction process of polysaccharides from Fu Brick tea and its antioxidation in vitro and effect on growth of probiotics

对茯茶多糖提取工艺进行优化,并对茯茶多糖体外生物活性进行研究.在浸提时间90 min、超声时间30 min、超声功率180 W、料液比1∶30的条件下,茯茶多糖提取率最高可达3.69%.茯茶多糖体外活性研究表明,0.6 mg/mL的茯茶多糖溶液总还原力为0.68,对·OH清除率为23.76%;0.3 mg/mL的茯茶多糖溶液对DPPH自由基的清除率为70.03%.茯茶多糖对益生菌体外生长表现出明显的影响作用.2 mg/mL和4 mg/mL茯茶多糖对戊糖片球菌和凝结芽孢杆菌的生长具有显著的抑制作用(P<0.05);5 mg/mL的茯茶多糖显著促进凝结芽孢杆菌和乳酸乳球菌的生长(P<0.05).本研究获取茯茶多糖的最佳提取工艺;揭示茯茶多糖体外抗氧化活性及其对益生菌生长的影响,对于高附加值茯茶多糖食品开发奠定重要基础.

In this study,the extraction process of Fuzhuan tea polysaccharide was optimized by response surface methodology,and the biological activity of Fu Brick tea polysaccharide in vitro was studied.When the extraction time was 90 min,the ultrasonic time was 30 min,and the ultrasonic power was 180 W and the solid-liquid ratio was 1∶30,and the highest extraction rate of Fu Brick tea polysaccharide was 3.69%.The total reducing power of Fu Brick tea polysaccharide solution of 0.6 mg/mL is 0.68,and the removal rate of OH is 23.76%.The DPPH radical scavenging rate of Fu Brick tea polysaccharide solution of 0.3 mg/mL was 70.03%.Fu Brick tea polysaccharide has obvious influence on probiotics in vitro.The growth of Pediococcus pentosaceus and Bacillus coagulans was significantly inhibited by 2 mg/mL and 4 mg/mL Fu Brick tea polysaccharide(P<0.05).5 mg/mL Fu Brick tea polysaccharide significantly promoted the growth of B.coagulans and Lactococcus lactis(P<0.05).In this study,the best extraction technology of Fu Brick tea polysaccharide was obtained.To reveal the antioxidant activity of Fu Brick tea polysaccharide in vitro and its effect on the growth of probiotics have important reference value for the research and development of high value-added Fu Brick tea health products.