基于AFLP和SSR分子标记的两个优良彩叶桂品种遗传多样性分析（英文）

The Genetic Relationship of Two New Colorful Sweet Osmanthus fragrans Cultivars Revealed by AFLP and SSR Markers

两种彩叶桂新品种"紫嫣公主"和"虔南桂妃"其叶片颜色渐变,观赏期较长,可在我国多地作园林观赏栽培。该文利用AFLP和SSR分子标记,以长叶木犀为对照品种,研究了"紫嫣公主"、"虔南桂妃"与其他桂花品种之间的亲缘关系。共筛选出55对AFLP引物和17对SSR引物,分别获得了1103个扩增片段和45个扩增片段,其中AFLP的多态性比率为92.29%,SSR的多态性比率为62.20%。在群体水平上,有效等位基因数变化范围为0.76~1.11,Shannon信息指数变化范围为0~0.11,揭示桂花品种的遗传基础较窄。遗传相似性分析表明"紫嫣公主"与其他桂花品种的相似性系数在0.61~0.80之间,"虔南桂妃"与其他桂花品种的相似性系数在0.57~0.67之间。UPGMA聚类结果表明,"紫嫣公主"可能属于银桂品种群;但"虔南桂妃"单独聚为一类,遗传变异较大。研究结果为种质资源保护和新品种选育提供了新的分子依据。

Osmanthus fragrans is well-known as an ornamental and agricultural plant noted for its unique fragrance.We investigated the genetic relationships between two new colorful O.fragrans cultivars,Ziyan Gongzhu and Qiannan Guifei,and their relationships with other O.fragrans using amplified fragment length polymorphism(AFLP)and simple sequence repeat(SSR),compared to the Changye Muxi species.55 AFLP primer pairs and 17 SSRs were screened;1103 and 45 amplification products were produced,of which 92.29%of the AFLP and 62.20%of the SSRs were polymorphic.At the population level,the number of effective alleles varied from 0.76 to 1.11,and the Shannon index(I)ranged from 0 to 0.11,indicating a narrow genetic base of O.fragrans cultivars.The genetic similarity varied from 0.61 to 0.80 between Ziyan Gongzhu and other O.fragrans cultivars,and 0.57 to 0.67 in the Qiannan Guifei and other cultivars.The unweighted pair group method with arithmetic mean(UPGMA)clustering revealed that Ziyan Gongzhu may belong to the O.fragrans Albus group.However,the Qiannan Guifei cultivar was clustered into a group,showing that it has great genetic variation.These results provide new molecular evidence for germplasm resources protection,and new cultivars for breeding.