稻瘟病菌分泌蛋白MoCDIE2诱导植物细胞死亡的功能分析

Functional Analysis of Secreted Protein MoCDIE2 Inducing Plant Cell-Death in Magnaporthe oryzae

由稻瘟病菌(Magnaporthe oryzae)引起的稻瘟病是全球最严重的植物真菌病害之一。稻瘟病菌通过分泌效应蛋白进入与植物相互作用界面或转运到植物细胞内,抑制寄主植物的免疫防卫反应,使病原菌成功侵染。通过农杆菌介导的异源表达策略,筛选到能引起非寄主植物烟草细胞死亡的候选效应蛋白MoCDIE2(Cell Death-Inducing Effector)。序列分析表明:MoCDIE2基因编码一个蓖麻毒素B凝集素蛋白;系统发育树构建结果表明MoCDIE2同源蛋白保守存在于丝状真菌中;利用基因敲除的方法获得MoCDIE2的敲除突变体,结果表明MoCDIE2的敲除突变体在菌丝生长和致病性方面与野生型菌株Guy11没有明显差异。

Rice blast,caused by the fungus Magnaporthe oryzae,is one of the most destructive plant diseases around the world.M.oryzae secretes many effector proteins into the interface or plant cells to suppress host immune defense response,thus helping the fungus to infect plant successfully.Using agrobacterium-mediated transient expression strategy,we obtained one putative effector protein MoCDIE2(Cell Death-Inducing Effector)that induces cell death in non-host plant Nicotiana benthamiana.Sequence alignment result indicated that MoCDIE2 gene encoded a ricin B lectin protein.Phylogenetic tree demonstrated that MoCDIE2 protein was conserved in many filamentous fungi.We used gene knock out method to generate MoCDIE2 knock out mutant,and the result showed that there were no significant differences between wild type strain Guy11 and mutants of MoCDIE2 knockout in mycelia growth and pathogenicity.