PCR-膜芯片技术在牦牛及犏牛肉制品的真假鉴定中的应用

Application of PCR-membrane Chip Technology in the Identification of Yak and Yak-cattle Meat

旨在利用PCR-膜芯片技术快速高效的鉴定牦牛及犏牛肉制品的真假。采集样品(猪、山羊、黄牛、水牛、牦牛、鸡、鸭、兔、狗、真犏牛、假犏牛肉样各8个,19个肉干制品样品),提取并纯化DNA,利用11对特异性引物进行多重PCR,将扩增产物与含有12个探针的膜芯片反向点杂交,测试膜芯片的准确度及灵敏度,并鉴定市场上销售的牦牛肉制品真假。结果表明,膜芯片准确度高,特异性强,无交叉反应,灵敏度能达到0.1 ng,检测灵敏度高。通过鉴定市场上销售的假牦牛肉干制品几乎都以黄牛和水牛肉为原料制作。应用PCR-膜芯片技术可快速准确的鉴定出牦牛肉制品的真假,有利于打击假冒产品,维护市场平衡。

This paper aims to quickly and efficiently identify the authenticity of yak and yak-cattle meat products by PCR-membrane chip technology.We collected samples(8 fresh meat and 19 dry meat samples of fresh pig,goat,oxen,buffalo,yak,chicken,duck,rabbit,dog,and yak-cattle),extracted and purified DNA,used 11 specific primers to perform multiplex PCR.Further we hybridized the amplified products with a membrane chip containing 12 probes via reverse dot blot,measured the accuracy and sensitivity of the membrane chip,and identified the authenticity of the commercially available yak meat products.The results demonstrated that the membrane chip was of high accuracy,strong specificity,no cross-reaction,and high detection sensitivity(low to 0.1 ng).Detection results of the commercial fake yak meat products showed that almost all of them were made from yellow cattle and buffalo meat.In conclusion,the application of PCR-membrane chip technology can quickly and accurately identify the real and fake yak meat products,which is conducive to combating counterfeit products and maintaining market balance.