槟榔碱对颊黏膜成纤维细胞增殖及迁移的影响

Effects of arecoline on proliferation and migration of human buccal mucosal fibroblasts in vitro

目的通过观察槟榔碱诱发人颊黏膜成纤维细胞增殖、迁移能力以及微丝形态的改变,探讨槟榔碱在口腔黏膜下纤维性变(OSF)的致病途径。方法使用甲噻唑四唑氮(MTT)比色法、划痕法、激光共聚焦扫描显微镜检测不同浓度槟榔碱(5、10、20、40、80μg·mL^-1)对成纤维细胞增殖、迁移、微丝形态的影响。结果5、10、20μg·mL^-1的槟榔碱可轻微提高颊黏膜成纤维细胞增殖迁移能力及微丝聚合(P<0.05);40、80μg·mL^-1的槟榔碱抑制颊黏膜成纤维细胞增殖、迁移能力及微丝解聚(P<0.05)。结论槟榔碱改变颊黏膜成纤维细胞增殖、迁移能力及微丝形态分布,可能在口腔黏膜下纤维性变病理过程中起着重要的作用。

Objective The role of arecoline in the pathogenesis of oral submucous fibrosis(OSF)was explored by investigating the effects of arecoline on the cell proliferation,migration and filament actin of human buccal mucosal fibroblasts in vitro.Methods The methyl thiazolyl tetrazolium(MTT)assay,wound healing assay and laser scanning confocal microscopy were used to detect the effect of arecoline at different concentration(5,10,20,40,80μg·mL^-1)for human buccal mucosal fibroblasts proliferation,migration and filament actin.Results Cell proliferation,migration and polymerization of filament actin of human buccal mucosal fibroblasts in vitro were enhanced by arecoline at lower concentrations(5,10μg·mL^-1)(P<0.05),and inhibited by arecoline at higher concentrations(20,40,80μg·mL^-1)(P<0.05).Conclusion Arecoline may change the proliferation and migration of human buccal mucosal fibroblasts through polymerization of filament actin.