子宫内膜癌组织中Flotillin-1蛋白的表达情况及与患者临床特征和预后的关系

Expression of Flotillin-1 protein in endometrial cancer tissues and the relationship with patients’ clinical characteristics and prognosis

目的探讨子宫内膜癌组织中浮舰蛋白-1(Flotillin-1)的表达情况及与患者临床特征和预后的关系。方法收集150例子宫内膜癌患者的子宫内膜癌组织及其对应的癌旁正常组织(距肿瘤边缘4 cm)。采用免疫组织化学染色法检测子宫内膜癌组织及癌旁正常组织中Flotillin-1蛋白的表达情况,分析子宫内膜癌组织中Flotillin-1蛋白表达情况与患者临床特征及预后的关系。结果子宫内膜癌组织中Flotillin-1蛋白的阳性表达率为36.0%(54/150),明显低于癌旁正常组织的68.0%(102/150),差异有统计学意义(P﹤0.01)。不同年龄的子宫内膜癌患者子宫内膜癌组织中Flotillin-1蛋白的阳性表达率比较,差异无统计学意义(P﹥0.05);高+中分化、无淋巴结转移、国际妇产科联盟(FIGO)分期为Ⅰ期、浸润深度≤1/2的子宫内膜癌患者子宫内膜癌组织中Flotillin-1蛋白的阳性表达率均高于低分化、有淋巴结转移、FIGO分期为Ⅱ~Ⅲ期、浸润深度﹥1/2的子宫内膜癌患者,差异均有统计学意义(P﹤0.05)。单因素分析结果显示,低分化、有淋巴结转移、FIGO分期为Ⅱ~Ⅲ期、浸润深度﹥1/2以及Flotillin-1阴性表达患者的中位生存期均短于高+中分化、无淋巴结转移、FIGO分期为Ⅰ期、浸润深度≤1/2及Flotillin-1阳性表达的患者,差异均有统计学意义(P﹤0.05);多因素Cox比例风险回归分析结果显示,低分化、有淋巴结转移、FIGO分期为Ⅱ~Ⅲ期、浸润深度﹥1/2以及Flotillin-1阴性表达均是影响子宫内膜癌患者预后的独立危险因素(P﹤0.01)。结论Flotillin-1蛋白在子宫内膜癌组织中的表达水平较低,且其表达水平与子宫内膜癌患者的临床特征有关,对于子宫内膜癌的发生发展具有重要的临床意义。

Objective To explore the expression of Flotillin-1 protein in endometrial cancer tissues and the relationship with patients’clinical characteristics and prognosis.Method The endometrial cancer tissues and surrounding normal tissues(4 cm away from tumor margin)were collected from 150 cases of endometrial cancer.The expression of Flotillin-1 protein was detected by immunohistochemistry in all tissue specimens,and its relationship with the clinical characteristics and prognosis was analyzed.Result The positive expression rate of Flotillin-1 protein in endometrial cancer tissues was 36.0%(54/150),which was significantly lower compared to the 68.0%(102/150)in surrounding normal tissues,and the difference was of statistical significance(P<0.01).The expression of Flotillin-1 protein showed no significant difference among patients with different age(P>0.05);but it was significantly higher in endometrial cancer patients with high or moderate differentiation,negative lymph node metastasis,International Federation of Gynecology and Obstetrics(FIGO)stage I,and depth of invasion≤1/2 compared with those who had lower differentiation,positive lymph node metastasis,FIGO stage II-III,depth of invasion>1/2,and the difference was statistically significant(P<0.05).Univariate analysis showed that,poor tumor differentiation,lymph node metastasis,FIGO stage II-III,depth of invasion>1/2 and negative expression of Flotillin-1 were associated with significantly shorter median survival time compared to those with high and moderate differentiation,negative lymph node metastasis,FIGO stage I,depth of invasion≤1/2 and positive Flotillin-1 expression,demonstrating statistically significant difference(P<0.05);multivariate Cox proportional hazard regression model analysis revealed that,poor differentiation,lymph node metastasis,FIGO stage II-III,depth of invasion>1/2 and negative Flotillin-1 expression were independent risk factors for the prognosis of endometrial cancer(P<0.01).Conclusion The expression of Flotillin-1 protein in endometrial cancer tissues is relatively low,and is significantly related to the clinical characteristics of endometrial cancer patients,thus indicating the essential clinical significance for the occurrence and development of endometrial cancer.