陆地棉转录因子GhWRKY72基因克隆及表达分析

Cloning and expression analysis of GhWRKY72 gene in Gossypium hirsutum L.

为研究WRKY转录因子在棉花中的功能及分子特征,本试验以陆地棉(Gossypium hirsutum L.)‘中棉19’为试验材料,克隆获得了转录因子基因GhWRKY72,并对其进行生物信息学及表达模式分析。结果表明:GhWRKY72开放阅读框为1578 bp,编码525个氨基酸;序列比对发现其氨基酸序列与海岛棉GbWRKY72转录因子基因同源性高达89.23%;在高盐(NaCl)或干旱(甘露醇)非生物胁迫下,GhWRKY72基因均可做出响应,尤其在盐胁迫条件下,该基因表现上调表达趋势;同时GhWRKY72基因的表达受茉莉酸(JA)、水杨酸(SA)和乙烯(ACC)诱导及黄萎病病菌(Verticillium dahliae)处理的影响,其中ACC、SA和黄萎病病菌处理均能诱导GhWRKY72上调表达。本研究可为理解WRKY基因家族在棉花抗逆和抗病过程中的作用机制提供参考。

To study the function and molecular characteristics of WRKY transcription factor in cotton,the GhWRKY72 transcription factor gene was cloned from upland cotton(Gossypium hirsutum L.),and its bioinformatics and expression patterns were further analyzed.The results showed that the open reading frame of GhWRKY72 was 1578 bp,which encoded 525 amino acid polypeptides.Sequence alignment showed that the amino acid sequence and the similarity of the island cotton GbWRKY72 transcription factor were as high as 89.23%.Under the abiotic stress treatment of salt(NaCl)and drought(mannitol),it was found that GhWRKY72 gene was involved in the response of the above two abiotic stresses,especially under salt stress,the gene showed up-regulated expression.At the same time,the expression pattern of GhWRKY72 gene was also induced by jasmonic acid(JA),salicylic acid(SA),acetyl-CoA carboxylase(ACC)and Verticillium dahliae.The results could provide a reference for understanding the mechanism of action of the WRKY gene family in cotton stress and disease resistance.