HBB rs35755129基因多态性与广西红水河流域长寿人群血糖血脂水平的相关性

Correlation between HBB rs35755129 polymorphism and blood glucose and lipid levels in longevity population in Hongshui River Basin of Guangxi in China

目的:探讨广西红水河流域长寿人群HBB rs35755129基因多态性与血糖血脂水平的相关性。方法:选取广西红水河流域人群303例,其中长寿组107例,年龄(93.46±3.09)岁;老年组68例,年龄(75.47±4.44)岁;年轻组128例,年龄(49.18±9.20)岁。使用PCR-Sanger测序对HBB启动区rs35755129位点进行基因分型,统计分析该位点多态性对空腹血糖(FBG)以及血脂指标的影响。结果:长寿组与老年组和年轻组间胆固醇(TC)、高密度脂蛋白(HDL)、载脂蛋白A(Apo A)和载脂蛋白B(Apo B)存在差异性(P<0.05)。总体人群中AA基因型空腹血糖高于GG/AG基因型,三酰甘油(TG)低于GG/AG基因型(P<0.05);年轻组AA基因型空腹血糖及三酰甘油高于GG/AG基因型(P<0.05)。整体男性AG/GG基因型三酰甘油水平高于AA基因型(P<0.05),女性AG/GG基因型空腹血糖低于AA基因型,Apo B高于AA基因型(P<0.05);长寿组男性AG/GG基因型低密度脂蛋白(LDL)低于AA基因型(P<0.05);年轻组男性AG/GG基因型空腹血糖低于AA基因型,三酰甘油高于AA基因型(P<0.05)。结论:HBB rs35755129基因型多态性与广西红水河流域人群血糖和血脂水平具有一定的相关性,但受性别因素影响,且在不同年龄寿限中发挥调控作用的基因型可能有所不同。

Objective:To explore the relationship between the polymorphism of HBB rs35755129 and blood glucose and lipid levels in longevity population in Hongshui River Basin of Guangxi.Methods:303 cases of Guangxi Hongshui River Basin population were selected and divided into longevity group([93.46±3.09]years old,n=107),elderly group([75.47±4.44]years old,n=68)and young group([49.18±9.20]years old,n=128).The genotypes of HBB were classified by PCR-Sanger sequencing,and the effects of polymorphism of the gene locus on fasting blood glucose(FBG)and lipid indexes were analyzed.Results:Cholesterol(TC),high-density lipoprotein(HDL),apolipoprotein A(Apo A)and apolipoprotein B(Apo B)in longevity group were lower than those in the elderly group and the young group(P<0.05).In overall,the fasting blood-glucose level of subjects with AA genotype was higher than those with GG/AG genotype,and triglyceride(TG)was lower than those with GG/AG genotype(P<0.05).In the young group,the expression level of locus on FBG and TG were higher than GG/AG genotype(P<0.05).Males AG/GG genotype carriers in total population presented higher TG level than those with AA genotype(P<0.05),while females AG/GG genotype carriers presented lower FBG level than AA genotype,and higher Apo B level than AA genotype(P<0.05).Males AG/GG genotype carriers in longevity group presented lower low-density lipoprotein(LDL)level compared with that in the AA carriers(P<0.05).In the young group,the total FBG level of AG/GG genotype carriers lower than AA genotype,while TG was higher than AA genotype(P<0.05).Conclusions:The polymorphism of HBB rs35755129 genotype is related to blood glucose and blood lipid,but it is influenced by gender factor.The genotype that plays a regulatory role in different life span may be different.