胃癌组织中IFN-γ、IL-4和SOX-2蛋白表达及其与幽门螺杆菌感染的相关性分析

The expression of interferon-γ, interleukin-4, and SRY-related HMG box gene 2 in gastric cancer and their correlations with helicobacterpylori infection

目的:探讨胃癌组织中γ-干扰素(IFN-γ)、白细胞介素-4(IL-4)、性别决定相关基因簇-2(SOX-2)蛋白表达情况及其与幽门螺杆菌(HP)感染的相关性。方法:选取其中胃癌样本67例(胃癌组)、胃炎样本85例(胃炎组)和胃溃疡样本73例(溃疡组),采用免疫组化法检测各组样本的IFN-γ、IL-4和SOX-2蛋白表达情况,并分析其阳性率与HP感染的关系。结果:胃炎组HP阳性率(91.8%,78/85)高于胃癌组(79.1,53/67)和溃疡组(74.0%,54/73),差异有统计学意义(P<0.05);胃癌组与溃疡组HP阳性率差异无统计学意义(P=0.475)。免疫组化染色显示,IFN-γ蛋白在胃癌组呈强阳性表达,在胃炎及溃疡组呈弱阳性表达;IL-4及SOX-2蛋白在胃癌组呈弱阳性表达,在胃炎及溃疡组呈强阳性表达。胃癌组IFN-γ蛋白阳性率高于胃炎组和溃疡组,差异有统计学意义(P<0.05);胃癌组IL-4和SOX-2蛋白阳性率低于胃炎组和溃疡组,差异有统计学意义(P<0.05)。胃癌HP感染与组织中SOX-2蛋白表达呈负相关关系(r=-0.732,P<0.05),与IFN-γ和IL-4蛋白表达无显著相关性。结论:胃癌的发生和发展可能与胃黏膜组织中IFN-γ蛋白表达上调、IL-4和SOX-2蛋白表达下调有关;HP感染可能通过影响SOX-2蛋白表达促进胃癌发生。

Objective:To analyze the expression of interferon-γ(IFN-γ),interleukin-4(IL-4),and SRY-related HMG box gene 2(SOX-2)in gastric cancer and their correlations with helicobacter pylori(HP)infection.Methods:A total of 67 cases of gastric cancer(gastric cancer group),85 cases of gastritis(gastritis group),and 73 cases of gastric ulcer(ulcer group)were included.The protein expression of IFN-γ,IL-4,and SOX-2 was detected by immunohistochemistry,and the relationship between their positive rates and HP infection was analyzed.Results:The positive rate of HP in gastritis group(91.8%,78/85)was significantly higher than that in gastric cancer group(79.1%,53/67)and ulcer group(74.0%,54/73),with statistically significant differences(P<0.05).There was no significant difference in the positive rate of HP between gastric cancer and ulcer groups(P<0.05).The expression of IFN-γprotein was strongly positive in gastric cancer group,weakly positive in gastritis and ulcer groups.Protein expression of IL-4 and SOX-2 was weakly positive in gastric cancer group,but strongly positive in the gastritis and ulcer groups.The positive rate of IFN-γprotein in gastric cancer group was higher than that in gastritis group and ulcer groups(P<0.05).HP infection in gastric cancer was negatively correlated with SOX-2 protein expression in tissues(r=﹣0.732,P<0.05),and was not significantly correlated with IFN-γand IL-4 protein expression.Conclusions:There may be relationship between occurrence and development of gastric cancer and the up-regulated protein expression of IFN-γand the down-regulated protein expression of IL4 and SOX-2,and HP infection might promote the occurrence of gastric cancer by affecting the expression of SOX-2 protein.