微孔板法检测婴幼儿配方乳粉中维生素B12的含量

Detection of vitamin B12 in infant formula milk powder by microporous plate method

目的建立用微孔板检测婴幼儿配方乳粉中维生素B12的方法。方法基于微生物原理的检测方法,缩小样品的培养体积。样品经提取后,分装到96孔细胞培养板中,在培养基中加入0.05%的菌悬液,混匀后分配到含有样品和标准品的微孔中,培养后直接用酶标仪测定吸光度值。结果本方法标准曲线相关系数0.9987,加标回收实验回收率在92.7%~98.7%之间,重复性实验相对标准偏差在1.03%~1.73%之间, 6种不同含量的样品同时用GB 5413.14-2010方法和本方法检测维生素B12,检测结果无显著性差异。结论该方法快速,操作简单,稳定性高,检测成本低,可大批量样品同时检测,适合测定婴幼儿配方乳粉中维生素B12的含量。

Objective To establish a method for detection of vitamin B12 in infant formula milk powder by microporous plate. Methods Based on the principle of microbial detection methods, reduce the training volume of the sample. The samples were extracted and packed into a 96-well cell culture plate, added 0.05% bacterial suspension to the B12 assay medium, after mixing, it was distributed to micropores containing samples and standard, after culture, the absorbance value was determined by enzyme standard instrument. Results The standard curve correlation coefficient was 0.9987, the recovery rates of the method were in the range of 92.7%-98.7%, the relative standard deviations of repetitive experiments were in the range of 1.03%-1.73%, six samples of different content were simultaneously tested with GB 5413.14-2010 method and this method for vitamin B12, there was no significant difference in the test results. Conclusion The method is rapid, easy to operate, high stability, low detection cost, easy to detect large quantities of samples at the same time, suitable for the determination of vitamin B12 content in infant formula milk powder.