高效液相色谱法测定保健食品中丙氨酰-酪氨酸的含量

Determination of alanyl tyrosine in health products by high performance liquid chromatography

目的建立高效液相色谱法测定保健食品中丙氨酰-酪氨酸含量的分析方法。方法样品经一级水超声提取,采用Agilent C18柱(150 mm×4.6 mm,2.7μm)进行分离,以乙腈-水溶液为流动相进行等度洗脱,采用高效液相色谱法检测,外标法定量。结果很好地分离了丙氨酰-酪氨酸,在浓度0.01~0.05mg/mL之间呈现良好的线性关系,平均回收率在95%~105%之间,相对标准偏差(relative standard deviation,RSD)为1.0%。结论该方法准确、灵敏度高、重现性好,适用于保健食品中丙氨酰-酪氨酸含量的测定。

Objective To establish a method for the determination of alanyl tyrosine in health products by high performance liquid chromatography.Methods Samples were ultrasonically extracted with Primary water solution,separated by Agilent C18 column with acetonitrile-Primary water solution as the mobile phase for gradient elution,which was detected by HPLC and quantified by external standard method.Results Alanyl tyrosine could be separated well,it had a good linear relationship within 0.01-0.05 mg/mL range,the recovery was 95%-105%,RSD was 1.0%.Conclusion This method is accurate,sensitive and reproducible.It is suitable for the determination of alanyl tyrosine in health foods.