小檗碱调节翼状胬肉成纤维细胞增殖能力的机制研究

Mechanism of berberine on the proliferation of pterygium fibroblasts

目的:探讨小檗碱对体外培养翼状胬肉成纤维细胞增殖能力的影响及可能的作用机制。方法:通过对手术获取的翼状胬肉组织进行培养,获取翼状胬肉成纤维细胞。采用不同终浓度(0、20、40、80μmol/L)小檗碱对细胞进行诱导,检测小檗碱诱导后翼状胬肉成纤维细胞凋亡水平、线粒体膜电位和凋亡相关因子mRNA与蛋白表达水平变化。结果:小檗碱能够以剂量依赖的方式提高体外培养翼状胬肉成纤维细胞线粒体去极化水平、细胞凋亡比率、促凋亡基因Bax和Bad mRNA与蛋白的表达水平,降低Bcl-2 mRNA和蛋白的表达水平。结论:小檗碱可能通过提高体外培养翼状胬肉细胞线粒体去极化水平诱导胬肉细胞发生凋亡。

AIM:To investigate the effect of berberine on proliferation of in vitro pterygium fibroblasts and its possible mechanism.METHODS:Pterygium fibroblast obtained from cultured pterygium tissue after surgical excision.Pterygium fibroblasts cells apoptosis level,mitochondrial membrane potential and apoptosis-related factors mRNA and protein expression levels were detected after the induction of berberine with different final concentrations(0,20,40,80μmol/L).RESULTS:Berberine increased the mitochondrial depolarization level,apoptosis rate,expression level of pro-apoptotic gene Bax,Bad mRNA and protein,and decreased the expression level of Bcl-2 gene mRNA and protein in vitro pterygium fibroblasts cells in a dose-dependent manner.CONCLUSION:Berberine may induce in vitro cultured pterygium cell apoptosis by increasing mitochondrial depolarization.