预知子化学成分的分离与鉴定

Isolation and structure identification of chemical constituents from the fruits of Akebiae quinata

研究预知子中的非三萜类化学成分。综合运用大孔吸附树脂HP-20、Sephadex LH-20、硅胶柱色谱、反相硅胶柱色谱及高效液相等色谱方法进行分离纯化,利用波谱学方法对得到的化合物进行结构鉴定。从预知子甲醇提取物中分离鉴定了14个非三萜类化合物:酪醇(1)、咖啡酸(2)、原儿茶酸(3)、对羟基苯甲酸(4)、阿魏酸(5)、红景天苷(6)、羟基酪醇葡萄糖苷(7)、反式松柏苷(8)、紫丁香苷(9)、木通苯乙醇苷B(10)、3-咖啡酰基奎宁酸(11)、4-咖啡酰基奎宁酸(12)、5-咖啡酰基奎宁酸(13)、1,5-二-O-咖啡酰基奎宁酸(14)。其中,化合物5~10、12~14为首次在预知子中得到。

To study the non-triterpenoid chemical constituents of the fruits of Akebiae quinata.Fourteen compounds were isolated and purified by various chromatography methods,including macroporous resin HP-20,Sephadex LH-20,silica gel column and RP-18 column.Their structures were identified by spectroscopic methods as tyrosol(1),caffeic acid(2),protocatechuic acid(3),p-hydroxybenzoic acid(4),ferulic acid(5),salidroside(6),hydroxytyrosol glucoside(7),trans-coniferin(8),syringin(9),calceolarioside B(10),3-O-caffeoylquinic acid(11),4-O-caffeoylquinic acid(12),5-O-caffeoylquinic acid(13)and 1,5-di-O-caffeoylquinic acid(14).Among them,compounds 5-10 and 12-14 were isolated from the fruits of Akebiae quinata for the first time.