白肉灵芝水提物对衰老大鼠皮肤的作用研究

Effect of the aqueous extracts of Ganoderma leucocontextum on aging rats skin

为研究白肉灵芝水提物(Ganoderma leucocontextum aqueous extracts,GLAE)对D-半乳糖致衰大鼠皮肤的影响,用D-半乳糖构建衰老大鼠模型,同时分别给予不同剂量的(0、50、100和200 mg/kg)的GLAE处理,利用生物显微技术观察皮肤组织结构的变化,ELISA法检测Ⅰ、Ⅱ型胶原蛋白含量,彗星电泳检测细胞DNA损伤,定量PCR检测基因MAP3K5、TGF-β1和PLOD2的表达水平。结果显示与模型组比较,GLAE干预后,大鼠皮肤病理改变明显减轻;皮肤细胞DNA损伤程度显著降低,Ⅰ、Ⅱ型胶原蛋白含量显著升高;基因MAP3K5、TGF-β1表达上调,基因PLOD2的表达下调。GLAE可增加胶原蛋白含量、抑制皮肤细胞DNA损伤、调节MAP3K5、TGF-β1和PLOD2基因的表达而发挥延缓皮肤衰老的作用。

The aim of this study was to investigate the effects of the aqueous extracts of Ganoderma leucocontextum on the skin of D-galactose-induced aging rats.Aging rat models were made by D-galactose,and then treated continuously with different doses(0,50,100,200 mg/kg)of GLAE.The changes of skin histological structure were observed by bio-microscopy.ELISA was used to determine the contents of typeⅠand typeⅡcollagen.The cell DNA damage and gene MAP3K5,TGF-β1 and PLOD2 expressions were detected by comet assay and quantitative PCR,respectively.The results showed that compared to model group,administration of GLAE could obviously alleviate skin pathological changes.The levels of cell DNA damage were markedly reduced,the contents of typeⅠand typeⅡcollagen were markedly increased.The expressions of gene MAP3K5 and TGF-β1 were up-regulated,while expressions of gene PLOD2 were statistically down-regulated.Based on these results,it was concluded that GLAE could delay skin aging process through enhancing collagen contents,inhibiting skin cell DNA damage,regulating expressions of gene MAP3K5,TGF-β1 and PLOD.