基于UPLC-Q-TOF-MS对野马追内酯F在大鼠体内代谢产物的分析

Analysis of metabolites of eupalinolide F in rats based on UPLC-Q-TOF-MS

采用超高效液相色谱-四级杆-飞行时间质谱系统分析野马追内酯F的质谱裂解规律及其在大鼠体内的代谢转化过程。采用Phenomenex Luna-C 18(2 mm×100 mm,3μm)色谱柱,流动相为乙腈-0.1%甲酸水溶液,梯度洗脱,流速0.2 mL/min,柱温30°C,进样体积2μL;动态背景扣除触发信息关联采集进行MS/MS数据采集;结合PeakView、MetabolitePilot软件进行代谢产物分析鉴定。野马追内酯F经过水解、氧化、还原、甲基化等I相代谢及葡萄糖醛酸化、硫酸酯化、谷胱甘肽结合等II相反应,形成系列相关代谢产物共55种。该方法高效灵敏,明确了野马追内酯F在大鼠体内的代谢转化规律,为中药野马追药效物质基础、新药研发及临床应用等提供科学参考。

Ultra-high performance liquid chromatography-four-stage rod-time-of-flight mass spectrometry(UHPLC-TOF-MS)was used to analyze the mass spectrometric decomposition and metabolic transformation of eupalinolide F in rats.Phenomenex Luna-C 18(2 mm×100 mm,3μm)column was used.The mobile phase was acetonitrile-0.1%formic acid aqueous solution,gradient elution,flow rate was 0.2 mL/min,column temperature was 30°C,injection volume was 2μL.The dynamic background subtraction trigger information correlation acquisition was used for MS/MS data acquisition.The metabolites were analyzed and identified by combining PeakView and MetabolitePilot software.Eupalinolide F undergoes phase I metabolism such as hydrolysis,oxidation,reduction,methylation,etc.,through the combination of glucuronidation,sulfuric acid esterification and glutathione II and multiple biotransformation pathways,formed a series of 55 related metabolites.The method is highly efficient and sensitive,and clarifies the metabolic transformation process of the eupalinolide F in rats,and provides scientific reference for the traditional Chinese medicine Eupatorium lindleyanum drug-effect material basis,new drug research and development and clinical application.