利用陶瓷膜技术精制丹酚酸B及其降糖活性的研究

Study on the purification of salvianolic acid B by using ceramic membrane and its hypoglycemic activity

为进一步优化制备丹参总酚酸的节能降耗工艺,本研究利用陶瓷膜技术,提高丹参中总酚酸和丹酚酸B的产率、并研究了丹酚酸B的降糖活性。通过考察水提醇沉法、30%乙醇提取法及陶瓷膜超滤法对丹参总酚酸提取的产率,利用不同陶瓷膜孔径对其水提液进行超滤,对该过程工艺参数进行研究,并采用HepG2细胞评价其降糖活性。本研究发现陶瓷膜技术能够显著提升丹参总酚酸的产率及其丹酚酸B的含量,且效率明显优于其它两种现有工艺,对丹酚酸B的降糖研究发现胰岛素抵抗模型成功,降糖活性显著。因此,本研究所采用陶瓷膜技术,能够显著提高丹参总酚酸的产率及其丹酚酸B的含量,且工艺步骤简单,上样量大,为丹参总酚酸的提取制备提供了一条节能降耗的工艺技术,以及开发丹酚酸B为降糖药物奠定工艺及前期实验基础。

In order to further optimize the energy saving and consumption reduction process for the preparation of total salvianolic acids,a ceramic membrane technology was developed and used to refine the yields of total salvianolic acids and salvianolic acid B.The hypoglycemic activity of salvianolic acid B was also evaluated using HepG2 cell model.The yield of total salvianolic acid was extracted by methods of water extraction and alcohol precipitation method,30%alcohol extraction and ceramic membrane ultrafiltration.Moreover,different apertures of ceramic membrane were used to evaluate efficiency of ultrafiltration,and HepG2 cell model was used to evaluate hypoglycemic activity.The results showed that ceramic membrane technology could significantly improve the yield of total salvianolic acid and salvianolic acid B,and the efficiency was significantly better than the other two methods.The insulin resistance model was successfully established to evaluate hypoglycemic activity of salvianolic acid B,which exhibit obvious hypoglycemic activity.Using ceramic membrane technology can significantly improve the yield of total salvianolic acid and the content of salvianolic acid B,and the process was simple and the sample quantity was well,it is vital that providing an energy saving technology for the extraction and preparation of total salvianolic acid,and could be candidates for anti-diabetic agents.