LDOC1及NF-κB p65在甲状腺乳头状癌中的表达及意义

Expression and significance of LDOC1 and NF-κB p65 in papillary thyroid carcinoma

目的探讨亮氨酸拉链下调因子1(LDOC1)和核转录因子(NF-κB)p65在对甲状腺乳头状癌(PTC)中的表达及临床意义。方法收集2011年1月至2014年1月间在广元市中医院病理科保存的110例PTC、55例结节性甲状腺肿和43例甲状腺瘤组织蜡块;采用免疫组化SP法检测组织中LDOC1和NF-κB p65蛋白表达。随访PTC患者术后复发情况,Kaplan-Meier法绘制无复发生存曲线。结果PTC组织LDOC1蛋白阳性率为30.91%(34/110),明显低于结节性甲状腺肿和甲状腺瘤;NF-κB p65蛋白阳性率为54.55%(60/110),明显高于结节性甲状腺肿和甲状腺瘤组织,差异有统计学意义(P<0.05)。PTC组织中LDOC1和NF-κB p65共阳性13例,共阴性29例,呈负相关(r=-0.219,P=0.021)。LDOC1表达与肿瘤直径、TNM分期有关;NF-κB p65表达肿瘤直径、包膜侵犯、淋巴结转移和TNM分期有关。LDOC1阴性表达者无复发生存率为69.74%(53/76),低于LDOC1阳性表达者(P=0.032)。NF-κB p65阳性表达者无复发生存率为65.0%(39/60),低于NF-κB p65阴性表达者(P<0.05)。Logistic多因素回归模型结果显示,LDOC1阴性表达(OR=7.802,95%CI=3.895~11.739)和NF-κB p65阳性表达(OR=11.167,95%CI=7.605~21.980)是影响PTC术后复发的独立预后因素(P<0.05)。结论PTC中LDOC1低表达,NF-κB p65高表达,两者联合有望成为预测PTC术后复发的指标。

Objective To investigate the expression and clinical significance of leucine zipper down-regulated factor 1(LDOC1)and nuclear transcription factor(NF-κB)p65 in thyroid papillary carcinoma(PTC).Methods From January 2011 to January 2014,110 cases of PTC,55 cases of nodular goiter and 43 cases of thyroid tumor tissue wax block were collected from the pathology department of Guangyuan hospital of traditional Chinese medicine.The expressions of LDOC1 and NF-κB p65 were detected by immunohistochemical staining method.The recurrence of PTC patients was followed up,and Kaplan-Meier method was used to draw the recurrence free survival curve.Results The positive rate of LDOC1 protein in PTC was 30.91%(34/110),which was significantly lower than that in nodular goiter and thyroid tumor;the positive rate of NF-κB p65 protein was 54.55%(60/110),which was significantly higher than that in nodular goiter and thyroid tumor(P<0.05).In PTC tissues,LDOC1 and NF-κB p65 were positive in 13 cases,negative in 29 cases,showing a negative correlation(r=-0.219,P=0.021).LDOC1 expression was related to tumor diameter and TNM stage,NF-κB p65 expression was related to tumor diameter,capsule invasion,lymph node metastasis and TNM stage.The recurrence free survival rate of LDOC1 negative expression was 69.74%,lower than that of LDOC1 positive expression(P=0.032).The recurrence free survival rate of NF-κB p65 positive patients was 65.0%,lower than that of NF-κB p65 negative patients(P<0.05).The results of Logistic regression model showed that the negative expression of LDOC1(OR=7.802,95%CI=3.895-11.739)and the positive expression of NF-κB p65(OR=11.167,95%CI=7.605-21.980)were independent prognostic factors(P<0.05).Conclusion In papillary thyroid carcinoma,LDOC1 was low and NF-κB p65 was high.The combination of the two is expected to be a predictor of PTC recurrence.