高效液相色谱法同时测定赤芍中没食子酸、没食子酸丙酯和柚皮素的含量

Simultaneous determination of gallic acid,propyl gallate and naringenin in Paeoniae Rubra Radix by HPLC

目的:建立一种同时测定赤芍中没食子酸、没食子酸丙酯和柚皮素三种活性成分的高效液相色谱方法。方法:采用ODS-C18色谱柱(250 mm×4. 6 mm,5μm),流动相采用甲醇-0. 1%乙酸水溶液,梯度洗脱;体积流量为1. 0 m L/min;检测波长为270 nm,柱温30℃。结果:没食子酸、没食子酸丙酯、柚皮素分别在5~250μg/m L浓度范围内具有良好的线性关系(R2> 0. 999)。检测方法有良好的精密度和回收率。测定1 g赤芍生药中含有没食子酸、没食子酸丙酯、柚皮素分别为1. 867、0. 561和0. 093 mg。结论:建立的高效液相色谱法能同时准确测定赤芍中没食子酸、没食子酸丙酯、柚皮素三个活性成分的含量,稳定可靠,可用于赤芍药材的质量控制。

Objective:To establish a high performance liquid chromatography(HPLC)method for simultaneous determination of the contents of gallic acid,propyl gallate and naringenin in Paeoniae Rubrathe Radix.Methods:The analysis of three compounds was performed on a 30℃thermostatic ODS-C18 HPLC column(250 mm×4.6 mm,5μm),with the mobile phase comprising of methanol-0.1%acetic acid flowing at 1.0 mL/min in a gradient elution manner.And the detection wavelength was set at 270 nm.Results:For three compounds,gallic acid,propyl gallate,and naringenin,good linear relationship could be obtained within the concentration ranges of 5-250μg/mL(R2>0.999),respectively.Results also indicated the good precision of the analytical method and good accuracy within the optimal recovery range.It was shown that 1 g of the raw material of Paeoniae Rubrathe Radix contained 1.867 mg of gallic acid,0.561 mg of propyl gallate,and 0.093 mg of naringenin,respectively.Conclusion:This method was able to accurately determine the contents of three active compounds in Paeoniae Rubrathe Radix,and could be used for the quality control of Paeoniae Rubrathe Radix.