胰腺癌患者外周血CD4^+T亚群细胞因子及转录因子的表达及其临床意义

Expression of cytokines and regulator transcription factors of CD4^+ subsets in patients with pancreatic cancer and its clinical significance

目的:探讨胰腺癌患者外周血中CD4^+T亚群细胞因子及转录因子表达。方法:收集27例胰腺癌患者和30例健康者外周血标本,ELISA法检测两组血清中IFN-γ、IL-2、IL-4、IL-17、转化生长因子(TGF)-β及IL-10含量。实时荧光定量PCR检测两组血清中相关转录因子T细胞表达的T盒(T-box expressed in T cells,T-bet)、GATA结合蛋白3(GATA-binding factor 3,Gata-3)、视黄酸相关的孤儿受体γt (RAR-related orphan receptor gamma t,RORγt)及叉头框蛋白p3(Forkhead box protein P3,Foxp3)表达。结果:与对照组相比,胰腺癌组外周血中细胞因子IFN-γ、IL-2水平明显降低,T-bet mRNA表达下降(P均<0. 05); IL-4、IL-17、TGF-β及IL-10水平明显升高,Gata-3 mRNA、RORγt mRNA及Foxp3 mRNA表达明显升高(P均<0. 05)。结论:胰腺癌患者外周血中Th1类细胞因子及转录因子表达下降,Th2、Th17和Tregs类细胞因子及转录因子表达升高; Th2、Th17及Treg细胞可能协同促进胰腺肿瘤疾病的进展。

Objective:To investigate the cytokines and master regulator transcription factors of CD4+subsets in pancreatic cancer patients.Methods:Specimens of peripheral blood were collected from 27 pancreatic cancer patients and 30 healthy controls.The serum levels of IFN-γ,IL-2,IL-4,IL-17,TGF-βand IL-10 were determined by ELISA.The mRNA expression level of T-bet,Gata-3,RORγt and Foxp3 were detected by real time-PCR.Results:Compared with healthy controls,the level of IFN-γ,IL-2 and T-bet mRNA were markedly decreased in pancreatic cancer patients(P<0.05),but the level of IL-4,IL-17,TGF-β,IL-10 and mRNA expression level of Gata-3,RORγt and Foxp3 were significantly increased in pancreatic cancer patients(all P<0.05).Conclusion:Pancreatic cancer patients showed lower levels of Th1 cytokines and master regulator transcription factors,higher levels of Th2,Th17 and Tregs cytokines and master regulator transcription factors;Th2,Th17 and Treg cells may synergistically promote the development of pancreatic cancer disease.