厌氧氨氧化污泥异养反硝化的影响因素分析

RESEARCH ON INFLUENCING FACTORS ON HETEROTROPHIC DENITRIFICATION OF ANAMMOX SLUDGE

以无机基质培养的厌氧氨氧化污泥为对象,研究污泥投量、DO、BOD 5/N和粒径等因素对其反硝化降解NO-3-N活性的影响。通过高通量测序技术获得细菌的种群结构状况,其中主要的厌氧氨氧化菌为Candidatus Brocadia fulgida和Candidatus Brocadia caroliniensis。批式实验结果表明:DO是厌氧氨氧化污泥异养反硝化最重要的限制性因素,ρ(DO)=0.12 mg/L是实现反硝化反应的界限;碳源浓度对厌氧氨氧化污泥异养反硝化的影响较小,碳源充足时,厌氧氨氧化污泥的异养反硝化活性受细菌数量限制,碳源不足时,混合菌群也可以充分利用原水碳源反硝化;从粒径角度分析得出,相对于底物扩散,微量氧对厌氧氨氧化污泥反硝化的限制更大。

In this paper,anaerobic ammonia-oxidized sludge cultured in inorganic substrates was focused to study the effects of sludge dosage,DO,BOD 5/N,and particle size on NO-3-N denitrification activity.First,the population structure of the bacteria was obtained by high-throughput sequencing technology,which was found to include anaerobic ammonia-oxidizing bacteria Candidatus Brocadia fulgida and Candidatus Brocadia caroliniensis.The results of batch experiments showed that:DO was the most important limiting factor for heterotrophic denitrification in anaerobic ammonium oxidation sludge.DO concentration of 0.12 mg/L was the limit for denitrification reaction.The effect of carbon source concentration to the denitrification of anammox sludge was quite limited.With sufficient carbon source,the heterotrophic denitrification activity of anammox sludge was limited by the number of bacteria.When the carbon source was insufficient,the mixed flora could fully utilize the raw water carbon source for denitrification.Compared with the substrate diffusion in the particle,the limitation from trace oxygen to the denitrification of anammox ammonia sludge was more powerful.