水稻OsPLATZ14基因启动子的克隆及表达分析

Cloning and Expression Analysis of OsPLATZ14 Promoter in Rice (Oryza sativa L.)

【目的】研究pOsPLATZ14的结构及时空表达模式,对深入研究OsPLATZ14基因功能、了解PLATZ转录因子在水稻生长发育进程的作用具有重要意义。【方法】利用BDGP、FPROM及Cister预测分析pOsPLATZ14大小,以水稻日本晴基因DNA为模板扩增pOsPLATZ14;应用PlantCARE分析序列中的顺序作用元件,构建pOsPLATZ14::GUS载体,转化水稻获得转基因植株;通过GUS组织化学染色法分析pOsPLATZ14在水稻中的时空表达特征。【结果】经PCR扩增获得pOsPLATZ14长度为1 899 bp,该区域含有光信号、逆境响应及激素应答等多种顺式作用元件。pOsPLATZ14驱动的GUS报告基因在水稻种子萌发期,苗期根、茎、叶,抽穗期的根、茎、叶、花穗、小花、叶夹角、茎结合部位、根茎过渡区及成熟期种子均有明显表达。【结论】pOsPLATZ14为组成型启动子,其下游调控基因OsPLATZ14可能在水稻生长发育过程中起重要作用。

【Objective】 In order to further understand the function of OsPLATZ14 and the role of PLATZ transcription factor in rice growth and development, the structure and spatiotemporal expression pattern of pOsPLATZ14 were studied.【Method】 The length of pOsPLATZ14 was predicted by using the online software of BDGP, FPROM, and Cister. The cisacting regulatory elements of pOsPLATZ14 were analyzed based on the PlantCARE database. pOsPLATZ14 was isolated from the genomic DNA of Nipponbare, then pOsPLATZ14::GUS vector was constructed and applied for the rice callus transformation. Using GUS histochemical assay, the spatiotemporal expression characteristics of pOsPLATZ14 were revealed.【Result】 The pOsPLATZ14 was determined to be 1 899 bp in length containing several cis-elements which were associated with the light signaling, stress and hormone response. The GUS reporter gene driven by the promoter was significantly expressed at the stages of seed germination, the seedling stage of roots, stems and leaves, in the heading period of roots, stems, leaves, flower spikes, florets, leaf angle, stem-stem junctions, root-stem junctions, as well as the seed maturation.【Conclusion】 pOsPLATZ14 was the constitutive promoter and its downstream regulatory gene OsPLATZ14 might play an important role in the growth and development of rice.