花生GSTs家族基因的全基因组分析

Comprehensive Genome-wide Analysis of GSTs Gene Family in Peanut

谷胱甘肽转移酶(glutathione-S-transferase,GST)是一类重要的多功能超家族酶,普遍存在于植物体中,作为配体或结合蛋白,在调控植物的生长发育、解除外界毒素作用过程起到重要作用。为全面了解花生GSTs基因家族的结构特性,本论文通过生物信息分析技术对花生GST基因家族进行全基因组鉴定和表达特性分析,结果表明,在花生全基因组中一共有163个GSTs基因,其中A组基因中含有76个,B组基因中含有87个。基因结构和发育进化树将花生GST基因分为6类:Tau、Theta、Lamda、EF1Bγ、Phi和DHAR,花生GST家族基因结构进化相对保守,但各基因的内含子数量及长度有较大差异,其中EK5R9的外显子数最多,达19个;而K7JNV的内含子最长,约18 kb。MEME分析发现,花生GST家族基因中存在9个保守域,各基因中的保守结构域为6~8个不等。染色体定位显示,花生GSTs家族基因在花生A、B染色体组上呈不均匀分布,在A02、A03、A07、A09、B02、B03、B05、B08和B09号染色体上存在1~2个基因簇。表达分析发现,只有80个花生GST基因具有组织表达差异,其中6个的组织表达有极显著差异。本研究为GSTs基因的功能研究及花生抗逆性提高提供了理论依据。

Glutathione-S-transferase(GST)is ubiquitous in plants,as an important multifunctional superfamily protein for plant growth and development,release of external toxins,and acts as ligands or binding proteins.In this study,a total 163 GST family genes were identified from peanut(A.duranensis with 76 and A.ipaensis with 87)genome,and classified into six subfamilies:Tau,Theta,Lamda,EF1Bγ,Phi and DHAR.These subfamilies showed significant difference in length and number of introns.And EK5R9 with the 19 exons is the most than other genes.K7JNV show the longest intron,about 18kb.Nine conserved motifs were identified through the online MEME analysis and all the peanut GSTs contain 6-8 motifs.Chromosome localization analysis revealed that the highly uneven distribution of peanut GST genes across the peanut genome on 20 chromosomes.And there are 1-2 gene clusters on chromosome A02,A03,A07,A09,B02,B03,B05,B08 and B09.Transcript profiling of 80 peanut GST genes showed the tissue-specific expression,and six of them showed the significant difference in expression level.This study provides a theoretical basis for the study of peanut using GSTs gene to improve stress resistance and GSTs gene function.