仙灵脾改善小鼠睡眠功效成分的分离鉴定

Isolation and identification of epimedium in improving sleep function of mice

仙灵脾提取物经大孔树脂粗分,利用小鼠助眠及自主活动模型进行筛选。活性分离组分经MCI、ODS层析柱进一步筛选分离有效组分得4个单体成分。经核磁共振结构鉴定,4个单体分别为:朝藿定C、朝藿定、淫羊藿苷、淫羊藿次苷。经动物试验筛选得出朝藿定C和淫羊藿苷有效提高小鼠入睡率、延长睡眠时间及减少活动次数和站立次数,并且朝藿定C效果更优。结果表明:仙灵脾具有改善睡眠作用的主要功效成分为朝藿定C,淫羊藿苷也具有较好助眠功能。

Epimedium extracts was roughly separated by macroporous resin,and screened by mouse sleep aid and autonomic activity model.The active separation component is further screened by MCI and ODS chromatography column to separate the effective components to obtain 4 monomer ingredients.Identified by nuclear magnetic resonance structure,the four monomers were:epimedin C,epimedin B,icariin,icarisid.The results showed that epimedin C and icariin effectively increased the rate of sleep in mice,prolonged the sleep time,and reduced the number of activities and standing times.And the effect of epimedin C is better.The main functional component of epimedium with improved sleep is epimedin C,and icariin also has a good sleep aid function.