马铃薯抗氧化活性成分研究

Study on antioxidant active components of potatoes

分析了宁夏地区种植的8种马铃薯总酚、总黄酮含量,并采用高效液相色谱法测定马铃薯中酚酸种类及其含量;通过体外抗氧化评价模型比较8种马铃薯对超氧阴离子、羟基自由基、DPPH自由基、ABTS自由基清除能力及FRAP铁还原能力。结果表明:黑美人总酚含量最高,为400.38 mg/100 g,夏波蒂总酚含量最低,为233.93 mg/100 g;费乌瑞它黄酮含量最高,为107.39 mg/100 g,庄薯3号黄酮含量最低,为33.17 mg/100 g;所有品种马铃薯的主要酚酸物质均为绿原酸,其中黑美人含量最高(91.46%);绿原酸含量与酚酸、黄酮含量呈极显著正相关性(P<0.01);酚酸含量与ABTS自由基清除能力相关性最大;黄酮含量与羟基自由基清除能力呈极显著负相关(P<0.01);绿原酸含量与ABTS自由基清除能力相关性最大。

The content of total polyphenols and flavonoids in eight varieties of potatoes grown in Ningxia was analyzed,and the type and content of phenolic acids in potatoes were determined by high performance liquid chromatography(HPLC).The scavenging capacity of 8 varieties of potatoes against superoxide anions,hydroxyl groups,DPPH,ABTS and ferric iron reduction capacity of FRAP were compared by in vitro antioxidant evaluation model.The results were shown as follows.The highest polyphenol content in Heimeiren was 400.38 mg/100 g,and the lowest in Xiabodi was 233.93 mg/100 g.The highest content of flavonoids was 107.39 mg/100 g in Fiverite,and the lowest in Zhuangshu 3 was 33.17 mg/100 g.Chlorogenic acid was the main kind of phenolic acids in all varieties of potatoes,and Heimeiren had the highest account(91.46%).It was found that chlorogenic acid content was significantly positively correlated with phenolic acids and flavonoids content(P<0.01).The correlation between phenolic acids content and ABTS scavenging ability was the highest,and flavonoid content was significantly negatively correlated with hydroxyl radical scavenging ability(P<0.01).The content of chlorogenic acid had the greatest correlation with ABTS scavenging ability.