GPX3在胃癌中的表达及临床意义

Clinical significance of expression of glutathione peroxidase 3 in gastric cancer

背景谷胱甘肽过氧化物酶3(glutathione peroxidase 3,GPX3)在胃癌(gastric cancer,GC)中低表达,但其表达与GC患者预后联系尚不明确.目的分析GPX3基因在GC中的表达模式及预后价值.方法首选,利用Oncomine数据库分析GPX3基因在GC及正常胃黏膜组织中的表达情况;利用KM Plotter数据库分析GPX3的表达对GC患者的预后进行分析.其次,运用TCGA数据集对GPX3的表达模式和预后价值进行验证.最后,通过免疫组织化学法检测含有90例胃腺癌及其癌旁组织的组织芯片对上述结果进一步验证.结果利用Oncomine数据库分析,发现GPX3基因在GC组中表达显著低于正常胃黏膜组织(P<0.05).利用KM Plotter数据库分析,发现GC组织中GPX3低表达与患者总体生存率显著相关(P<0.05).利用TCGA数据集对GPX3的表达模式和预后价值进行验证,提示GPX3低表达与患者GC患者总体生存率显著相关(P<0.05).通过对90例GC及其癌旁组织的组织芯片研究分析发现,GPX3在癌组织中显著低表达(P=0.037),GPX3低表达与患者的年龄、性别、肿瘤临床分期无关,但与GC患者总体生存率显著相关(HR=0.48,95%CI:0.28-0.85,P=0.019).结论GPX3在GC中呈低表达,且与预后显著相关.

BACKGROUND Glutathione peroxidase 3(GPX3)expression is downregulated in gastric cancer(GC),but the relationship between GPX3 expression and prognosis in this malignancy is yet unknown.AIM To explore the expression pattern and prognostic value of GPX3 in GC.METHODS GPX3 expression was analyzed based on the Oncomine database.The prognostic value of GPX3 in GC patients was investigated using the KM Plotter database.To validate the expression pattern and prognostic value of GPX3,TCGA GC dataset was also analyzed.Finally,the expression pattern and prognostic value of GPX3 was evaluated by tissue microarray and immunohistochemistry in 90 GC patients.RESULTS Oncomine database analysis showed that GPX3 was significantly down-regulated in GC tissues compared with normal tissues(P<0.05).Data from the KM Plotter database showed that GPX3 low expression was significantly related with overall survival(P<0.05).TCGA dataset analysis also showed that GPX3 low expression was an indicator of better prognosis(P<0.05).Tissue microarray and immunohistochemistry showed that GPX3 was significantly down-regulated in GC tissue(P=0.037).GPX3 expression was related with GC patient overall survival(HR=0.48,95%CI:0.28-0.85,P=0.019),rather than age,gender,and tumor clinical stage.CONCLUSION GPX3 is downregulated in GC,and GPX3 expression can be used to predict GC patients’prognosis.