正常食蟹猴不同适应时间视网膜电图的变化

Changes in the Electroretinogram in Normal Cynomolgus Monkeys atDifferent Adaptation Times

目的:研究明/暗适应时间对正常成年食蟹猴闪光视网膜电图(ERG)结果的影响。方法:实验研究。选取3只成年食蟹猴,分别于暗适应20、40、60 min后用国际临床视觉电生理会标准参数刺激,常规程序记录暗适应ERG,在暗适应40 min后,分别明适应1、5、10 min记录明适应ERG。采用单因素方差分析比较各时间段振幅与潜伏期变化。结果:不同暗适应时间,a、b波和震荡电位OPs的潜伏期差异均无统计学意义(F=0.052,P=0.949),暗适应时间小于40 min时,a、b波和OPs的振幅随暗适应时间增加而显著增大(F=50.800,P<0.001);暗适应40 min时,振幅达到最大,且不再随暗适应时间增加而增大(F=0.016,P=0.899)。明适应a、b波的潜伏期同样不受明适应时间的影响(F=0.980,P=0.381),a、b波的振幅在明适应5 min后达到最大(F=4.789,P=0.036)且随时间延长趋于平稳(F=0.135,P=0.717)。结论:在一定时间内,明/暗适应时间对食蟹猴闪光ERG结果影响较大,故对食蟹猴暗适应ERG记录的时间不宜少于40 min,而明适应ERG在5 min后即可记录。

Objective:To study the effects of light/dark adaptation times on flash electroretinograms in normal adult cynomolgus monkeys.Methods:In this experimental study,three adult cynomolgus monkeys were selected and stimulated with standard electroretinogram(ERG)parameters after dark adaptation for 20 min,40 min and 60 min.The scotopic-ERG was recorded using aroutine procedure.After dark adaptation for 40 min,the photopic-ERG was recorded for 1 min,5 min and 10 min.One-way ANOVA was used to compare the amplitude and latency.Results:There was no significant difference in the latencies of a and b waves and OPs in the scotopic-ERG(F=0.052,P=0.949).When the dark-adaption time was less than 40 min,the amplitudes of the a and b waves and OPs increased significantly as the adaptation time increased(F=50.800,P<0.001).When the dark-adaptation time was more than 40 min,the amplitude reached the maximum and no longer increased with time(F=0.016,P=0.899).For the photopic-ERG,the implicit times of the a and b waves were also unaffected by the length of the light-adaptation time(F=0.980,P=0.381).The amplitudes of the a and b waves reached their maximum after 5 minutes of light adaptation(F=4.789,P=0.036)and tended to be stable over time(F=0.135,P=0.717).Conclusions:In a certain period of time,the results of flash ERG of cynomolgus monkeys are greatly affected by bright/dark time.So,the dark-adaptation time for scotopic-ERG recording in cynomolgus monkeys should be at least 40 minutes,and the photopic-ERG are best recorded after 5 minutes of light adaption.