益生菌复配富铬酵母缓解2型糖尿病的作用机制涉及调节肠道菌群失调

Antidiabetic effects of probiotics compounded with chromium-rich yeast alleviate type 2 diabetes symptoms via regulating gut microbiota imbalance

本研究通过高脂饲料和腹腔注射链脲佐菌素STZ诱导2型糖尿病小鼠模型,实验小鼠随机分为5组:空白组、模型组、药物组、乳酸菌组、复合组。实验结果发现肠道菌群失调与2型糖尿病的发生发展存在着密不可分的关联。模型组小鼠短链脂肪酸含量和物种丰富度显著下降,部分有益菌丰度降低,而致病菌丰度显著提升。此外,药物组、乳酸菌组和复合组均在一定程度上改善了糖尿病小鼠的肠道菌群结构和功能。其中与乳酸菌组相比,复合组的保护效果更显著,更显著增加Lactobacillus的丰度,降低Coprococcus和Rikenellaceae-g_的丰度,同时短链脂肪酸含量被显著增加。综上,复合组对2型糖尿病小鼠肠道菌群失调具有最显著的改善作用。本研究将为益生菌复配剂应用于2型糖尿病的干预治疗提供重要参考依据。

This study explored the mechanism of probiotics with chromium-rich yeast on Type 2 diabetes in mice.The disease was induced by high-fat diet and intraperitoneal injection of STZ.The fifty mice were divided into five groups.The results showed that Type 2 diabetes is closely related to the imbalance of gut microbiota.The level of the short-chain fatty acids(SCFAs)and the diversity of intestinal microbiota in these mice were significantly reduced,the abundance of some beneficial bacteria was decreased and the abundance of pathogenic bacteria was increased.After intervention,the gut microbiota imbalance was alleviated in drug-treated group,probiotics-treated group and cocktailtreated(using mixture of probiotics and chromium-rich yeast)group.Compared with the probiotics-treated group,the cocktail group showed more significant protective effects,including increase in the abundance of Lactobacillus and SCFA levels,and decrease in glucose level and abundance of Coprococcus and Rikenellaceae-g.Therefore,mixture of probiotics and chromium-rich yeast had more effective and comprehensive protective effects on type 2 diabetes.This study provided a theoretical basis for microbial mixture treatment method as an intervention treatment for Type 2 diabetes.