乳腺癌易感基因相关蛋白1的表达与胃癌预后的相关性

Correlation between the expression of breast cancer susceptibility gene-related protein 1 and prognosis of gastric cancer

目的探讨胃癌组织中乳腺癌易感基因相关蛋白1(BAP1)的表达与胃癌病人临床病理因素和预后的关系。方法收集2012年1月至2016年1月云南省第三人民医院收治的87例手术切除的Ⅰ~Ⅲ期胃癌病人胃癌组织及其癌旁组织,应用免疫组化法检测全组胃癌和癌旁组织中BAP1蛋白的表达水平。在87例病人中选取32例,采用实时荧光定量聚合酶链反应(PCR)检测胃癌和癌旁组织中BAP1 mRNA的表达水平,分析BAP1的表达水平与临床病理特征和预后的关系。结果免疫组化结果表明BAP1在胃癌组织和癌旁组织中的高表达率分别为39.1%和66.7%,差异有统计学意义(χ2=13.290,P=0.000)。PCR检测结果显示癌组织BAP1 mRNA的表达明显低于癌旁组织[(1.195±0.470)比(3.399±0.891),t=15.320,P=0.000]。BAP1的表达与胃癌病人的肿瘤分化程度、浸润深度、淋巴结转移和TNM分期关系密切(P值分别为:0.043、0.021、0.008、0.001),Kaplan⁃Meier分析结果表明BAP1低表达组和高表达组病人5年生存率分别为27.6%和58.2%(χ2=6.392,P=0.012),低表达组病人预后不良。Cox回归多因素分析发现肿瘤浸润深度、TNM分期、BAP1表达水平是影响胃癌病人生存预后的独立危险因素(P<0.05)。结论BAP1在胃癌组织中呈低表达,与胃癌病人的预后密切相关。

Objective To study the expression of BAP1 protein levels in gastric cancer and its correlation with the clinical progno⁃sis of gastric cancer.Methods Gastric cancer and matched non⁃tumor paracancerous tissue specimens ofⅠ⁃Ⅲstage were collect⁃ed from 87 postoperative patients in the Third People’s Hospital of Yunnan Province between Jan.2012 and Jan.2016.The expres⁃sion of BAP1 protein in 87 gastric cancer specimens and paired adjacent non⁃tumor tissues were detected by immunohistochemistry.Thirty two out of the 87 patients were randomly selected,and real time quantitative PCR were used to examine the expression of BAP1 mRNA levels in the 32 paired fresh gastric cancer and adjacent non⁃tumor tissues.The relationship between the protein ex⁃pression and clinicopathological features was analyzed.Results The expression of BAP1 in gastric cancer tissues was significantly lower than that in adjacent non⁃cancerous tissues(60.9%vs.33.3%,χ2=13.290,P=0.000).BAP1 mRNA levels were lower than that in adjacent non⁃cancerous tissues[(1.195±0.470)vs.(3.399±0.891),t=15.320,P=0.000].Low expression of BAP1 was sig⁃nificantly correlated with differentiation,depth of tumor invasion,lymph node metastasis and TNM stage(P=0.043,0.021,0.008,0.001,respectively).A lower expression of BAP1 demonstrated significantly poorer overall survival in gastric cancer patients than in those with higher BAP1 expression(27.6%vs.58.2%,χ2=6.392,P=0.012).Multivariate analyses suggested that depth of tumor in⁃vasion,TNM stage and BAP1 expression were independent predictors for overall survival(P<0.05).Conclusion The expression of BAP1 in gastric cancer was down⁃regulated.As an independent risk factor,low expression of BAP1 indicates a poor prognosis for patients with gastric cancer.