黑果枸杞多糖对菌群人源化小鼠肠道微生物调节研究

Regulation Research of Lycium ruthenicum Polysaccharide on Gut Microbes in HFA Mice

[目的]以菌群人源化小鼠(HFA-小鼠)作为模型,研究不同浓度黑果枸杞多糖对HFA小鼠肠道微生物的影响。[方法]征集一名健康志愿者,通过接种志愿者的粪便悬液构建HFA小鼠模型,将40只HFA小鼠随机分为4组,黑枸杞多糖低剂量组(CT+LPL)灌胃枸杞多糖50 mg/kg,黑枸杞多糖中剂量组(CT+LPM)灌胃枸杞多糖100 mg/kg,黑枸杞多糖高剂量组(CT+LPH)灌胃枸杞多糖200 mg/kg,正常对照组(CT)每天灌胃同体积的生理盐水。连续灌胃21 d,试验期间记录各组小鼠的体重变化,第22天处死小鼠后,取标本进行各项指标检测。[结果]CT+LPM组小鼠体重低于CT组,呈显著性差异(P<0.05),CT+LPM组HFA小鼠肠道中乳酸杆菌和双歧杆菌数量较CT组均增加,肠杆菌和肠球菌数量均减少,差异显著(P<0.01),CT+LPM组HFA小鼠肠黏膜sIgA含量极显著高于CT组(P<0.01)。[结论]黑枸杞多糖能够改善HFA小鼠肠道菌群环境,具有益生元作用,也能促进HFA小鼠肠黏膜sIgA的分泌。

[Objective]To study the effects of different concentrations of Lycium ruthenicum polysaccharide on the gut microbes of HFA mice using the source-based mice(HFA-mice)as a research model.[Method]A healthy volunteer was recruited to construct a HFA mouse model by inoculating the volunteers’faecal suspension,40 HFA mice were randomly divided into four groups,with the Lycium ruthenicum polysaccharide low dose group(CT-LPL)infused with polysaccharide 50 mg/kg,and the Lycium ruthenicum polysaccharide medium dose group(CT-LPM)infused with polysaccharide 100 mg/kg,the high-dose group of Lycium ruthenicum polysaccharide(CT-LPH)infused with polysaccharide 200 mg/kg,the normal control group(CT)infused the stomach with the same volume of saline every day.The weight changes of each group of mice were recorded during the experiment,execution of the mice after 21 th day,the samples were tested for various indicators.[Result]Compared with the CT group,the weight of the mice in the CT-LPM group decreased significantly,showing significant differences(P<0.05),the number of Lactobacillus and Bifidobacterium in the intestines of the HFA mice in the CT-LPM group increased,the number of Enterobacteriaceae and Enterococcus decreased,and the difference was statistically significant(P<0.01),the content of intestinal mucosa sIgA in the CT+LPM group was significantly higher than CT group(P<0.01).[Conclusion]Lycium ruthenicum polysaccharide can improve the environment of intestinal microbe in HFA mice,which have a probiotic effect,and can also promote the secretion of sIgA in HFA mice.