Copan WASP全自动微生物前处理系统性能及其临床应用价值的评估

Evaluation of performances and clinical application of Copan WASP automated microbial pretreatment instrument

目的:评估Copan WASP全自动微生物前处理系统的工作性能,探讨其在临床中的应用价值。方法:配制标准菌株铜绿假单胞菌ATCC27853、大肠埃希菌ATCC25922、金黄色葡萄球菌ATCC25923和化脓链球菌ATCC19615混合悬液共16份,作为Copan WASP全自动微生物前处理系统工作性能评估之用,采集临床痰、中段尿标本各50份以验证该系统在临床中的应用效果。分别采用前处理系统(仪器法)及手工方法进行划线接种,24 h培养后观察其细菌生长情况,并采用统计软件SPSS 18.0对其生长的菌种数量、菌落计数及单个菌落分离数量进行比较。结果:Copan WASP全自动微生物前处理系统连续10块平板划线接种菌落计数为(1.07±0.21)×104 cfu/ml,与理论值差异无统计学意义(P=0.322>0.05),且无交叉携带污染。16份不同浓度混合菌悬液仪器法和手工法均可回收菌株数量分别为114和106种,2种方法间差异无统计学意义(P=0.251>0.05)。仪器法与手工法临床标本分离菌种数量的差异(痰:P=0.549;尿:P=0.639)及菌落计数的差异(痰:P=0.154;尿:P=0.895)均无统计学意义,检测单个菌落数量的差异无统计学意义[痰:(26.28±5.31)和(20.84±5.59)个,P=0.111>0.05;尿:(9.74±4.49)和(7.33±5.03)个,P=0.532>0.05]。结论:Copan WASP全自动微生物前处理系统的主要性能指标在重现性和交叉污染两方面均满足临床要求,分离菌种数量及菌落计数与手工法无明显差异,可应用于临床。应用该系统可提高菌落分离效率,减少再次分离次数,缩短报告时间。

Objective To evaluate the performances of Copan WASP automated microbial pretreatment instrument and explore its value of in clinical application.Methods A total of 16 suspensions were prepared with the standard strains Pseudomonas pyogenes ATCC27853,Escherichia coli ATCC25922,Staphylococcus aureus ATCC25923 and Streptococcus pyogenes ATCC19615 for evaluation of the instrument performances,and 50 clinical specimens of sputum and mid-stream urine collected from the First Affiliated Hospital of Air Force Medical University were inoculated by Copan WASP and manual respectively,for verification of the effectiveness of the instrument in clinical application.After 24 hours of culture,the bacterial growth was observed.The number of strains,colony counts and effective single isolates were compared using statistical software SPSS 18.0.Results The colony counts on 10 consecutive plates streaked with Copan WASP automated microbial pretreatment instrument was(1.07±0.21)×104 cfu/ml,which had no significant difference with the theoretical value of 10(P=0.322>0.05).There was no cross-carrying contamination observed.There were 114 and 106 strains recovered from 16 different concentrations of suspensions by Copan WASP and manual respectively that showed no significant difference in the number of strains between the two methods(P=0.251>0.05).Similarly,there were no statistically significant differences in the number of strains and colony counts in clinical samples between the two inoculations(Sputum,P=0.549 and P=0.154;Urine,P=0.639 and P=0.895).The numbers of effective single isolates by Copan WASP and manual operation were 26.28±5.31,20.84±5.59 in sputum samples(P=0.111>0.05)and 9.74±4.49,7.33±5.03 in urine samples(P=0.532>0.05),respectively.Conclusion The reproducibility and cross-contamination of Copan WASP automated microbial pretreatment instrument meet the clinical requirements.There are no significant differences in the number of strains and colony counts between the instrument and the manual method.It could be applied to clinical practice for improving the efficiency of colony separation,reducing the frequency of re-separation and shortening the time of test report.