HPLC法同时测定风寒感冒颗粒中苦杏仁苷、葛根素和橙皮苷的含量

Simultaneous determination of amygdalin, puerarin and hesperidin in Fenghan Ganmao Granules by HPLC

目的:建立高效液相色谱法(HPLC)同时测定风寒感冒颗粒中苦杏仁苷、葛根素和橙皮苷含量的方法。方法:采用Thermo BDS Hypersil-C 18色谱柱(4.6 mm×250 mm,5μm),以甲醇-水为流动相,梯度洗脱;柱温40℃。结果:苦杏仁苷、葛根素、橙皮苷线性范围分别为0.01923~0.7692μg(r=1.000)、0.02562~1.025μg(r=1.000)、0.006816~0.2726μg(r=0.9999);平均回收率分别为101.4%、101.6%和100.4%,RSD(n=9)分别为1.34%、1.42%和1.00%。测定样品5批,苦杏仁苷、葛根素、橙皮苷的测定结果分别为:批号180115:0.1741、0.1903、0.08474 mg·g-1;批号180723:0.3929、0.4433、0.1241 mg·g-1;批号20180301:0.6745、0.4961、0.3599 mg·g-1;批号20180921:0.7212、1.006、0.2712 mg·g-1;批号1810220:1.513、1.820、0.3405 mg·g-1。结论:该方法可用于风寒感冒颗粒的质量控制。

Objective:To establish an HPLC method for simultaneous determination of amygdalin,puerarin and hesperidin in Fenghan Ganmao Granules.Methods:The test was performed in a Thermo BDS Hypersil-C 18 column(4.6 mm×250 mm,5μm)under the gradient elution of methanol-water.The column temperature was 40℃.Results:The linearity ranges of amygdalin,puerarin and hesperidin fell into 0.01923-0.7692μg(r=1.000),0.02562-1.025μg(r=1.000)and 0.006816-0.2726μg(r=0.9999)respectively.The average recoveries of amygdalin,puerarin and hesperidin were 101.4%,101.6%and 100.4%with the RSDs(n=9)as 1.34%,1.42%and 1.00%respectively.The test results of amygdalin,puerarin and hesperidin in 5 batches of samples were respectively as follow:batch No.180115:0.1741,0.1903,0.08474 mg·g-1;batch No.180723:0.3929,0.4433,0.1241 mg·g-1;batch No.20180301:0.6745,0.4961,0.3599 mg·g-1;batch No.20180921:0.7212,1.006,0.2712 mg·g-1;batch No.1810220:1.513,1.820,0.3405 mg·g-1.Conclusion:The established method can be used for quality control of Fenghan Ganmao Granules.