外源性VD3缓解运动诱导的慢性疲劳综合症的机制

Mechanism of exogenous VD3 supplement alleviates the exercise-induced chronic fatigue syndrome

研究了补充VD3缓解运动诱导的慢性疲劳综合症(CFS)的机制。将80只小鼠随机分5组(n=16),即安静对照组(C组)、CFS造模组(CFS组)、VD3组又分为造模前组(bVD组)、过程中组(mVD组)和造模后VD干预组(aVD组)。造模、干预持续4周,全程行为学观察,4周后进行力竭测试和悬尾实验,再迅速采血并切取股四头肌组织。测定血睾酮、血清皮质酮及股四头肌组织的MDA、T-AOC、GSH-PX和T-SOD,采用Western Blotting测定SIRT1、SIRT3、ERK1/2、p-ERK1/2和MEK1/2。结果表明:与C组比较,CFS组小鼠打斗损伤严重,血睾酮及MEK、SIRT3显著降低(P<0.05),皮质酮、MDA、T-SOD、GSH-PX显著增加(P<0.05);与CFS组比较,VD干预组小鼠打斗损伤较轻,皮质酮、MDA显著降低(P<0.05),血睾酮、T-AOC、T-SOD、GSH-PX及MEK、SIRT1、SIRT3和p-ERK/ERK均显著增加(P<0.05)。外源性补充VD3可提高骨骼肌抗氧化能力,缓解运动诱发的CFS症状,其机制可能是通过激活MEKs/ERKs-SIRT1信号通路,降低MEK表达,增加ERK磷酸化水平,上调SIRT1和SIRT3表达而实现。

The exogenous VD3 supplement alleviates exercise-induced chronic fatigue syndrome(CFS)and its mechanism are investigated.80 male mice were randomly divided into 5 groups(n=16),named quiet control group(C),CFS model group(CFS),VD supplement group before(bVD),during(mVD)and after(aVD)modeling.The modeling and supplement continued 4 weeks while behavior observation were achived.After 4 weeks,the tail suspension test and the forced swimming test were implemented,then the blood and quadriceps were dissected rapidly.Serum testosterone and corticosterone content,as well as MDA,T-AOC,GSH-PX and T-SOD in the quadriceps were measured.Western blotting was used to measure SIRT1,SIRT3,ERK1/2,p-ERK1/2 and MEK1/2.Compared with C group,CFS group appeared traces of fighting,and the concentration of serum testosterone,MEK and SIRT3 decreased significantly(P<0.05)while cortisol concentration,MDA,T-SOD,GSH-PX increased significantly(P<0.05).Compared with CFS group,VD supplement group showed less traces of fighting and the concentration of serum testosterone,T-AOC,T-SOD,GSH-PX,MEK,p-ERK/ERK,SIRT1 and SIRT3 increased significantly(P<0.05)while cortisol,MDAdecreased significantly(P<0.05).Exogenous VD3 supplement can alleviate CFS potentially by activating the MEKs/ERKs-SIRT1 signal transduction axis and upregulating the expression of SIRT3.