不同乳杆菌缓解慢性酒精性肝损伤的作用比较

Comparison of the alleviation effects of different Lactobacilli on chronic alcohol-induced liver injury

该文探究不同乳杆菌对小鼠慢性酒精性肝损伤的缓解作用。利用C57BL/6小鼠,随机分为空白对照组、酒精模型组、阳性对照组和乳杆菌干预组。8周后,通过分析小鼠的血清转氨酶活性、肝脏氧化指标、血清内毒素含量、肝脏炎症因子表达、肠道紧密连接蛋白表达水平等,并进行肝组织病理学观察,从而比较不同乳杆菌对慢性酒精性肝损伤的缓解作用。结果表明,所选乳杆菌均能够一定程度地改善慢性酒精引起的小鼠肝损伤。其中,植物乳杆菌LP45(Lactobacillus plantarum 45,LP45)和鼠李糖乳杆菌L519(Lactobacillus rhamnosus 519,L519)效果最为显著。植物乳杆菌LP45和鼠李糖乳杆菌L519显著降低了慢性酒精引起的肝脏脂肪积累和氧化应激,抑制了血液中转氨酶和内毒素的增加,降低了肝脏中炎症因子的升高,并改善了肠道紧密连接蛋白表达的降低。因此,所选乳杆菌中,植物乳杆菌LP45和鼠李糖乳杆菌L519可以通过改善氧化应激和增强肠道屏障功能从而有效缓解小鼠慢性酒精性肝损伤。

In this study,the alleviation effects of different Lactobacillus strains on chronic alcohol-induced liver injury in mice were investigated.C57 BL/6 mice were randomly divided into control group,alcohol model group,positive control group and Lactobacillus strains intervention groups.After treatment for 8 weeks,serum enzymes,liver oxidative stress,serum endotoxin levels,expression of hepatic inflammatory cytokines and intestinal tight junctional protein were analyzed,and the pathological changes of liver tissues were observed.The selected Lactobacillus strains could improve the liver injury of mice caused by chronic alcohol to a certain extent.Among them,Lactobacillus plantarum 45(LP45)and Lactobacillus rhamnosus 519(L519)significantly reduced liver fat accumulation and oxidative stress caused by chronic alcohol,inhibited the increase of serum enzymes and endotoxin,lowered the elevation of inflammatory factors in the liver,as well as improved the decrease in intestinal tight junction protein expression.In conclusion,LP45 and L519 could effectively alleviate chronic alcohol-induced liver injury in mice by improving oxidative stress and intestinal barrier function.