基于生物信息学分析SOX12在口腔鳞状细胞癌中的表达及验证

The expression and biological function of SOX12 in oralsquamous cell carcinoma by bioinformatics analysis

目的通过生物信息学分析转录因子SOX12在口腔鳞状细胞癌(OSCC)中的表达,进一步探讨其与OSCC临床病理特征的关系,阐明SOX12对OSCC早期诊断及预后的影响。方法运用GEPIA数据库结合TCGA数据库分析SOX12在OSCC组织中的表达水平,并通过qRT-PCR进行体外验证;利用LinkedOmics数据库描述SOX12表达与OSCC临床病理学参数的相关性及对预后的影响;从String-DB数据库中分析SOX12在信号转导中的位置及与其关系密切的上下游基因。结果SOX12在OSCC中呈高表达,且与OSCC病理分级、T分期、N分期呈正相关;TCGA分析显示,SOX12的表达与OSCC患者术后总体生存率有关;与SOX12相互作用的蛋白有POU3F1、POU3F2、POU3F3、MYT1L、NRSN2等,主要参与神经元的分化和发育等功能。结论SOX12在OSCC中呈高表达,并与OSCC多个病理性指标相关。

Objective To investigate the expression of transcription factor SOX12 in oral squamous cell carcinoma(OSCC)by bioinformatics analysis and to clarify the connection between expression of SOX12 and clinicopathological features of OSCC,so as to elucidate the early diagnosis and prognostic significance of OSCC by SOX12.Methods This study used the GEPIA database in conjunction with the TCGA database to analyze the expression level of SOX12 in OSCC tissues and then verify it by qRT-PCR in vitro.The LinkedOmics database was used to describe the correlation between SOX12 expression and clinical pathological parameters of OSCC and its impact on prognosis.The location of SOX12 in signal transduction and its upstream and downstream genes related to it were analyzed by the String-DB database.Results SOX12 was highly expressed in OSCC and positively correlated with OSCC pathological grade,T stage,and N stage.The results of TCGA showed that high SOX12 mRNA expression was associated with overall survival in OSCC.The proteins interacting with SOX12 included POU3F1,POU3F2,POU3F3,MYT1L,and NRSN2,which were mainly involved in the differentiation and development of neurons.Conclusion The TCGA database analysis found that SOX12 is highly expressed in OSCC and is associated with multiple pathological indicators of OSCC.