枫杨醇提物对刀豆蛋白A诱导的免疫性肝损伤的保护作用

Protective effect of Pterocarya hupehensis Skan extract on the immune liver injury induced by concanavalin A

目的探究枫杨醇提物对刀豆蛋白A(conA)所致肝损伤的保护作用及机制。方法将40只6周龄C57BL/6雄性小鼠随机分为正常组、模型组及枫杨醇提物低、中、高剂量治疗组,每组8只。除正常组尾静脉注射生理盐水外,其余小鼠注射conA(12 mg/kg)复制免疫性肝损伤模型,治疗组在注射后立即灌胃给予相应浓度的枫杨醇提物,于12 h对各组小鼠进行摘眼球取血及解剖取肝组织进行病理学观察,采集并计算肝系数,检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的含量,以及肝组织中STAT-3的相对含量及磷酸化程度。结果HE染色显示,与正常组比较,模型组肝组织出现明显损伤,治疗组则明显改善。相较于模型组,各治疗组皆能降低肝系数及血清中ALT、AST、IL-6和TNF-α的含量(P<0.05或P<0.01)。模型组STAT-3较正常组磷酸化显著增高,枫杨醇提物治疗组则可明显抑制磷酸化。结论枫杨醇提物能有效治疗conA诱导的免疫性肝损伤,其机制可能与抑制STAT-3的磷酸化有关。

Objective To investigate the protective effect and mechanism of Pterocarya hupehensis Skan extract on liver injury induced by concanavalin A(conA).Methods Forty C57BL/6 male mice were randomly divided into 5 groups:normal group,model group,and groups of low-,medium-and high-dose extract,with 8 mice in each group.Except for the normal group that received intravenous injection of normal saline,the other mice were injected with conA(12 mg/kg)for replication of the immunological liver injury model.The treatment groups were intragastrically administered with corresponding concentrations of extract.After 12 hours,the mice were subjected to eyeball extraction and dissection of liver tissue to prepare HE stained sections.The liver index was collected and calculated.Serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were also detected.The content of STAT-3 and the level of phosphorylation in liver tissue were also tested.Results HE staining showed that the liver tissue of the model group was significantly damaged compared with the normal group,and that of the treatment groups were ameliorated.Compared with the model group,each treatment group had reduced liver index and serum ALT,AST,IL-6 and TNF-αlevels(P<0.05 or P<0.01).Significant phosphorylation of STAT-3 was observed in the model group,but it was inhibited in the treatment groups.Conclusion The extract of Pterocarya hupehensis Skan can effectively treat immunological liver injury induced by conA,which may be related to the inhibition of phosphorylation of STAT-3.