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受激辐射耗尽超分辨显微成像的研究进展及应用

Research Progress and Application of Stimulated Emission Depletion Super-Resolution Imaging
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摘要 荧光显微镜凭借其非接触、无损伤、可实时探测生物样品内部等优点,成为生命科学研究中不可或缺的工具,但是Abbe光学衍射极限的存在限制了其对亚细胞尺度生化过程的进一步研究。作为突破光学衍射极限的远场光学显微镜,受激辐射耗尽(Stimulated Emission Depletion,STED)超分辨荧光显微术,因其超高时空分辨率和三维层析能力,成为备受关注的新型成像和分析表征工具。本文结合我们课题组在STED显微成像研究方面的工作,综述了近年来STED显微成像技术的研究进展及其在细胞成像中的应用。 Fluorescence microscopy is an indispensable tool in life science research because of its non-contact,non-invasive,real-time detection of the interior of biological samples,but the existence of E.Abbe’s optical diffraction limit hinders the further study of subcellular physiological processes.As the earliest far-field optical microscopy that broke the optical diffraction limit,stimulated emission depletion(STED) super-resolution microscopy has become a new imaging tool which attracts great attention due to its ultra-high spatial-temporal resolution and three-dimensional tomographic capability.Based on our experience in the development and application of STED microscope,this paper reviews the recent advances in STED microscopy and its application in cell imaging.
作者 阮贺飞 吴亚运 袁景和 石彦 方晓红 RUAN He-fei;WU Ya-yun;YUAN Jing-he;SHI Yan;FANG Xiao-hong(Beijing National Research Center for Molecular Sciences,Key Laboratory of Molecular Nanostructure and Nanotechnology,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190;School of Medicine,Tsinghua-Peking Joint Center for Life Sciences,Tsinghua University,Beijing 100084)
出处 《分析科学学报》 CAS CSCD 北大核心 2019年第6期723-731,共9页 Journal of Analytical Science
基金 国家自然科学基金重点项目(No.21735006)
关键词 受激辐射耗尽显微镜 超分辨成像 荧光探针 细胞成像 衍射极限 Stimulated emission depletion microscopy Super-resolution imaging Fluorescence probe Cell imaging Diffraction limit
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