基于人血清白蛋白占位的内标型比率荧光探针检测抗癫痫药物盐酸噻加宾

Human Serum Albumin-Occupying-Based Ratio Fluorescent Probe for Determination of Anti-epileptic Drug Tiagabine Hydrochloride

本文提出了一种检测抗癫痫药物盐酸噻加宾(TGB)的内标型比率荧光探针(PDI-HSA-AC)。该探针以人血清白蛋白(HSA)为竞争结合反应载体,染料苝二酰亚胺衍生物(PDI)为荧光基团,茜素络合指示剂(AC)为内标物。通过对PDI、HSA、AC和TGB相互作用的研究,我们发现PDI的荧光能够被HSA猝灭,并且PDI结合在HSA的位点Ⅱ处,当存在TGB时,TGB竞争结合在HSA的位点Ⅱ,PDI被置换下来,从而实现了荧光恢复。此外,TGB的存在基本不影响结合在HSA位点Ⅰ处的AC的荧光信号,因此实现了对TGB的比率荧光法的检测。构建的荧光探针具有灵敏度高、选择性好以及检测方法简单、实验条件温和、检测迅速等优点,并且能够应用于实际尿液中TGB的检测。

An internal calibration-based ratio fluorescent probe PDI-HSA-AC was firstly proposed for the detection of anti-epileptic drug tiagabin hydrochloride(TGB).The probe consisted of human serum albumin(HSA) as the competitive binding reaction container,perylene diimide derivative(PDI) as the fluorescent report unit,and alizarin complexone(AC) as the internal standard substance.In the study of the interaction between PDI,HSA,AC and TGB,we found that the fluorescence of PDI can be quenched by HAS via binding to site Ⅱ of HSA.In the presence of TGB,TGB competitively bind to site Ⅱ and replace PDI of HSA.As a result,the fluorescence of PDI is recovered.In addition,the TGB does not affect the fluorescence signal of AC at the HSA site I,thus achieving the ratio fluorescence detection of TGB.The probe has the advantages of high sensitivity,good selectivity,simple detection method,mild experimental conditions,rapid detection,and can be applied to the detection of TGB in actual urine.