炎症细胞因子基因多态性与癌因性疲乏的关联研究

Correlation between inflammatory cytokine polymorphisms and cancer-related fatigue

目的探讨炎症细胞因子基因多态性与癌因性疲乏(CRF)的关联,为研究遗传易感性在CRF发生发展中所起的作用提供研究基础。方法肺癌患者242例,其中癌因性疲乏组162例,非癌因性疲乏组80例。通过PCR-RFLP进行基因分型,分析基因型及等位基因频率在疲乏组与非疲乏组的分布情况及炎症细胞因子基因多态性与癌因性疲乏的关联性。结果TNFα-308G/A、IL-1β-511C/T基因型分布在两组间差异有统计学意义(P均<0.05)。多元logistic回归校正了年龄和性别等混杂因素后显示,相对于TNFα-308GG基因型患者,携带GA/AA基因型患者发生CRF的风险降低了74%(15%~92%);相对于IL-6-174GG基因型患者,携带GC/CC基因型患者发生CRF的风险降低了78%(0%~95%);在IL-1β-511C/T基因位点中,携带CC基因型的患者发生CRF的风险是TT基因型患者的3.96倍(95%CI 1.02-15.45)。结论携带TNFα-308GG、IL-6-174GG、IL-1β-511CC基因型的肺癌患者发生癌因性疲乏的风险明显增加。

Objective To explore the correlation between the inflammatory cytokine polymorphisms and cancer-related fatigue(CRF),and provide research basis for the investigation of genetic variation in the development and progression of CRF.Methods Totally 242 lung cancer patients were divided into CRF group(162)and non-CRF group(80).Genotyping was carried out according to the polymerase chain reaction restriction fragment length polymorphism(PCR RFLP),then the distribution of allele frequency and genotypes were analyzed,as well as the correlation between inflammatory cytokine polymorphisms and CRF.Results There was statistically significant difference between the distribution of TNFα-308 G/A and IL-1β-511 C/T genotypes(all P<0.05).After adjusting the confounding factors such as age and gender,multivariate logistic regression showed that patients with GA/AA genotype had a lower risk for CRF by 74%as compared with patients with TNFα-308 GG genotype(15%-92%);patients with GC/CC genotype had a lower risk by 78%in comparison with patients with IL-6-174 GG genotype(0%-95%);in the genetic locus of IL-1β-511 C/T,the patients with CC genotype were 3.96 times more likely to develop CRF than those with TT genotype(95%CI 1.02-15.45).Conclusion Patients with TNFα-308 GG,IL-6-174 GG and IL-1β-511 CC genotypes have a significantly increased risk of CRF.