糖尿病合并梅毒感染兔模型的梅毒病情变化

Changes of syphilis condition on diabetes mellitus combined with syphilis model in rabbits

目的动态监测糖尿病合并梅毒感染兔和单纯梅毒感染兔的梅毒血清学变化、睾丸肿胀程度及睾丸组织病理变化随病程发展的情况,并初步探讨其机制。方法以24只成年雄性新西兰兔为实验动物,分别纳入T、H、C组,每组8只,采用梅毒螺旋体接种兔睾丸构建单纯梅毒感染模型(T组),诱导剂诱导糖尿病再接种梅毒螺旋体构建糖尿病合并梅毒感染模型(H组),另设健康对照组(C组),采用甲苯胺红不加热血清试验(TRUST)和梅毒螺旋体颗粒凝集试验(TPPA)联合方式进行梅毒血清学监测,同时观察睾丸肿胀变化,对睾丸组织进行病理切片、染色,观测病理损伤情况。结果H组兔及T组兔在感染梅毒后的10 d左右TPPA呈阳性,并在实验的整个病程保持阳性,且两组的TRUST滴度自感染梅毒后随病程上升,3周左右达最高滴度,而后下降。H组与T组相比,实验过程除第1、4、13周滴度水平比较,差异无统计学意义(P>0.05),大部分时间两组滴度水平比较,差异有统计学意义(P<0.05);且两组最高滴度比较,差异有统计学意义(P<0.05)。同时,H组比T组在TRUST最高滴度时睾丸肿胀更明显,甚至出现明显水肿、溃烂及钙化现象,而T组仅表现出睾丸肿胀。病程结束,相比于C组,H组和T组睾丸组织均表现出多种炎性细胞浸润、明显的间质细胞增生及结节坏死现象;与T组兔相比,H组兔睾丸组织的病理变化更明显。结论与单纯梅毒感染兔相比,糖尿病合并梅毒感染兔出现了更为明显的梅毒感染症状,引起了更为严重的病理损伤,可见糖尿病的基础疾病加速了梅毒病程的发展。

Objective To dynamic monitor serological examination of syphilis,observe the degree of testicular swelling and detect pathological changes of testicular tissue of animal model of diabetes mellitus combined with syphilis with the development of disease course,then discuss its mechanism preliminarily.Methods A total of 24 adult male New Zealand rabbits were divided into group T,group H,group C,each group had 8 rabbits,rabbits in group T were established by vaccinating Treponema pallidum on rabbit testicular,the diabetes mellitus combined with syphilis(group H)group were established by inducing diabetes by inductor,then vaccinating Treponema pallidum,the normal control group(group C)were also established.Serological monitoring of syphilis were detected by using a combination of TPPA and TRUST,changes in testicular swelling were observed at the same time,in order to observe the pathological damage,testicular tissue were sectioned and stained with HE.Results TPPA was positive in group T rabbits and group H rabbits about 10 d after syphilis infection and remained positive throughout the course of the study.And degree drops of TRUST of them were rising until reach the highest titer about 3 weeks,and then fall.Compared with group T,titer levels in group H were significantly different during most of the experiment(P<0.05),except for the titer levels at week 1,4 and 13(P>0.05).Moreover,there was a significant difference in the highest titer between the two groups(P<0.05).Meanwhile,the testicular tissue in group H appeared obvious edema,ulceration and calcification,however,the group T did not behaved,Testicular swelling was more pronounced in group H than in group T at the highest titer.Compared with group C,the testicular tissues of group H and group T showed a variety of inflammatory cell infiltration,obvious interstitial cell hyperplasia and nodular necrosis at the end of the course.Compared with group T,the pathological changes of testicular tissues in group H rabbits were more significant.Conclusion Compared with syphilis infected rabbits,diabetes mellitus combined with syphilis infected rabbits have shown a more pronounced syphilis infection,causing more severe pathological damage,it could be seen that the basic disease of diabetes accelerates the development of syphilis course.