3项指标联合检测对儿童原发传染性单核细胞增多症的诊断价值

The diagnostic value of three indicator combined detection on primary infectious mononucleosis in children

目的探讨血浆人类白细胞抗原G(HLA-G)、可溶性白细胞抗原G(sHLA-G)、EB病毒(EBV)-DNA载量对儿童原发传染性单核细胞增多症(IM)的诊断价值。方法将60例IM患儿作为观察组,并以同期非IM的发热儿童60例作为对照组。检测并比较两组血浆HLA-G、sHLA-G、EBV-DNA载量,采用受试者工作特征(ROC)曲线分析HLA-G、sHLA-G、EBV-DNA载量及联合检测对儿童IM的诊断效能。结果观察组血浆HLA-G、sHLA-G、EBV-DNA载量均明显高于对照组,差异有统计学意义(P<0.05);ROC曲线分析显示血浆HLA-G、sHLA-G、EBV-DNA载量联合检测诊断儿童IM的曲线下面积(0.958)高于HLA-G(0.886)、sHLA-G(0.789)、EBV-DNA载量(0.904)单项检测。结论血浆HLA-G、sHLA-G、EBV-DNA载量水平可作为诊断儿童IM的重要指标,联合检测的特异度和准确度更高。

Objective To study the diagnostic value of plasma human leukocyte antigen G(HLA-G),soluble leukocyte antigen G(sHLA-G),Epstein-Barr virus-DNA(EBV-DNA)load on primary infectious mononucleosis(IM)in children.Methods A total of 60 children with primary IM were selected in observation group,and 60 non-IM children with fever in the same period were selected in the control group.The expression of plasma HLA-G,sHLA-G,EBV-DNA load in two groups were detected,and the diagnostic value of the primary IM in children was analyzed by ROC.Results The plasma HLA-G,sHLA-G,and EBV-DNA load in the observation group were significantly higher than those in the control group(P<0.05).ROC curve analysis showed that the area under the curve of combined detection of plasma HLA-G,sHLA-G,EBV-DNA load for diagnosis of IM(0.958)was higher than HLA-G(0.886),sHLA-G(0.789),and EBV-DNA load(0.904).Conclusion Plasma HLA-G,sHLA-G and EBV-DNA load could be used as important indicators for the diagnosis of IM in children.The combined detection has higher specificity and accuracy.