水力负荷与有机负荷协同作用对人工湿地微生物群落结构的影响

Synergistic effects of hydraulic loading and organic loading on microbial community structure in constructed wetlands

以单层基质结构(CW1)、3层基质结构(CW3)、6层基质结构(CW6)的水平潜流人工湿地系统为对象,考察水力负荷及有机负荷对不同人工湿地系统去除COD、总悬浮固体(TSS)效果的影响,同时利用高通量测序技术,分析高负荷条件下系统微生物群落结构。结果表明,不同水力负荷运行条件下,随着有机负荷的提高,CW1、CW3、CW6湿地系统对COD、TSS的去除率均表现出先升高后下降的趋势,总体看来,在相同运行条件下CW6湿地系统COD、TSS的去除率最高。群落结构分析表明,不同人工湿地系统微生物结构存在相似性,但湿地床体的基质结构会对微生物群落结构产生一定影响,总的来说分层填充的人工湿地系统微生物群落结构更为稳定,对污染物的去除效果更好。

The horizontal subsurface flow constructed wetland systems,with monolayer matrix structure(CW1),3 layers matrix structure(CW3),6 layers matrix structure(CW6)separately,were selected as research subject to investigate the effect of hydraulic and organic loading on COD and TSS purification efficiency.At the same time,microbial community structure of constructed wetland under the high loading conditions was analyzed by highthroughput sequencing technology.The results showed that under different hydraulic loading conditions,the removal efficiencies of COD and TSS increased first and then decreased with the increasing organic loadings.Basically,under the same conditions,CW6 achieved the highest COD and TSS removal efficiencies.The analysis of community structure showed that the microbial structure of different wetland systems was similar,while due to the different matrix structure of wetland bed,the microbial community structure had a certain impact.In general,the constructed wetland system with layered filling had a more stable microbial community structure and a higher removal effect of pollutants.