DR5在慢性乙型肝炎患者外周血单核细胞中的表达

Expression of DR5 on peripheral blood mononuclear cells in chronic hepatitis B patients

目的了解不同程度慢性乙型肝炎(CHB)患者外周血单核细胞(PBMC)中DR5分子的表达水平及其临床意义。方法收取2015年12月至2017年12月李惠利东部医院的CHB住院患者90例,分为轻度(30例)、中度(32例)、重度CHB组(28例),另外选择27名健康成年人作为健康对照组。采用荧光定量PCR技术和Western印迹法检测各组PBMC中DR5分子mRNA和蛋白的表达水平。多组间两两比较采用单因素方差分析中的Scheffe法。结果荧光定量PCR结果显示,轻、中、重度组CHB患者的PBMC中DR5 mRNA的表达即2^-△△Ct值分别为2.127、2.111、4.695,相比对照组均有显著增高(F=1.085,1.068,2.237,P<0.01或0.05),重度组中DR5 mRNA的表达显著高于轻度和中度组(F=1.025,1.073,P<0.05)。非加权线性趋势检验F=235.083,P<0.01。Western印迹法结果显示,与正常组和轻度组相比,DR5蛋白在中度组和重度组的灰度比值有显著升高(F=0.064、0.502、0.074和0.519,P均<0.01)。与中度组相比,重度组的灰度比值也显著升高(F=0.448,P<0.01)。非加权线性趋势检验F=408.832,P<0.01。结论DR5 mRNA及蛋白表达水平在CHB患者PBMC中显著升高,并且随疾病严重程度的增加呈现递增的趋势。

Objective To understand the expression of DR5 on peripheral blood mononuclear cells(PBMC)among different groups of chronic hepatitis B(CHB)patients and its clinical significance.Methods A total of 90 CHB patients in Ningbo Medical Center Lihuili Eastern Hospital during December 2015 and December 2017 were selected,and were divided into mild(30 cases),moderate(32 cases)and severe(28 cases)CHB groups.Other 27 healthy individuals were enrolled as control group.Real-time PCR and Western blot were used to detect the expression of DR5 mRNA and DR5 protein in different CHB groups,respectively.Scheffe model was used to compare the differences among different groups.Results Real-time PCR results showed that the expressions of DR5 mRNA in mild,moderate and severe CHB groups,namely,2^-△△Ct values of 2.127,2.111 and 4.695,were significantly higher than those in control group(F=1.085,1.068 and 2.237,P<0.01 or 0.05).The expression of DR5 mRNA in severe CHB group was significantly higher than that in mild or moderate group(F=1.025,1.073,P<0.05).Linear trend test showed F=235.083,P<0.01.The expressions of DR5 protein significantly increased in severe and moderate CHB group comparing with mild and control group(F=0.064,0.502,0.074,0.519,P<0.01).The expression of DR5 protein was also significantly higher in severe CHB group than that in moderate CHB group(F=0.448,P<0.01).Linear trend test showed F=408.832,P<0.01.Conclusions The expression levels of DR5 mRNA and protein in PBMC are significantly high in CHB patients,and show an increasing trend with the aggravation of hepatitis.