血管介入与阿司匹林、低分子肝素、降纤酶联合应用在小鼠脑缺血中的治疗作用

Therapeutic effects of vascular intervention combined with aspirin, low molecular weight heparin and defibrase on cerebral ischemia in mice

目的探究血管介入与阿司匹林、低分子肝素、降纤酶联合应用在小鼠脑缺血中的治疗作用。方法构建小鼠脑缺血模型,80只小鼠随机分为对照组和观察组(各40只),对照组采用阿司匹林、低分子肝素和降纤酶治疗,观察组小鼠采用血管介入联合阿司匹林、低分子肝素、降纤酶治疗;3个月后比较2组治疗前后收缩期峰值速度(Vs)、舒张末期速度(Vd)、神经功能缺损程度(NDS评分)及相关标记物蛋白(MMP-9、SOD、NLRP3)的表达水平。结果治疗3个月后与对照组比较,观察组中Vs值、Vd值、NDS评分、MMP-9、SOD、NLRP3蛋白表达水平明显降低(P<0.05)。与治疗前比较,观察组治疗3个月后上述指标明显降低(P<0.05),而对照组无明显变化(P>0.05)。结论颅内血管介入治疗可以有效地改善脑缺血小鼠的神经功能、改善血流障碍、降低氧化活性和炎症,效果优于常规药物联合治疗。

Objective To investigate the therapeutic effects of vascular intervention combined with aspirin, low molecular weight heparin and defibrase on cerebral ischemia in mice. Methods A mouse model of cerebral ischemia was constructed. Eighty mice were randomly divided into control group and observation group. The control group was treated with aspirin, low molecular weight heparin and defibrase. The observation group were treated with vascular intervention combined with aspirin, low molecular weight heparin and defibrase. Three months later, the peak systolic velocity(Vs), end-diastolic velocity(Vd), neurological deficit(NDS score), and related marker proteins(MMP-9, SOD, NLRP3) were compared between the two groups. Results After three months of treatment, the Vs value, Vd value, NDS score, MMP-9, SOD and NLRP3 protein expression levels in the observation group were significantly lower than those in the control group(P<0.05). Compared with before treatment, the above indicators were significantly lower after three months of treatment(P<0.05), but there was no significant change in the control group(P>0.05). Conclusion Intracranial vascular intervention could effectively improve the neurological function, improve blood flow disorder, reduce oxidative activity and inflammation in mice with cerebral ischemia. Its effect was superior to conventional drug combination therapy.